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首页> 外文期刊>Analytical Letters >Electrochemical Determination of Hydrogen Peroxide Using a Prussian Blue-Copper Modified Platinum Microelectrode
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Electrochemical Determination of Hydrogen Peroxide Using a Prussian Blue-Copper Modified Platinum Microelectrode

机译：普鲁士蓝铜修饰的铂微电极电化学测定过氧化氢

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摘要

Prussian blue modified with copper was electrodeposited on a platinum microelectrode for the amperometric determination of hydrogen peroxide at -50 mV. The Prussian blue-copper layer was stable for 2 h at pH 7.4 and a storage period of 2 months. The Prussian blue-copper modified microelectrodes were used to develop oxidase based microbiosensors for ethanol and lactate. Lactate and alcohol oxidases were immobilized by cross-linking with glutaraldehyde. No interferences were observed from possible interfering compounds up to a concentration of 0.5 mM. The calibration plot of the lactate microbiosensor was linear between 0.005 and 0.2 mM with a limit of detection of 0.0035 mM lactate. The linear dynamic range of the alcohol microbiosensor was between 0.05 and 2 mM and the limit of detection was 0.02 mM ethanol. The developed microbiosensors were used for the analysis of pickled cucumber juice, yogurt, and wine.
机译：用铜修饰的普鲁士蓝电沉积在铂微电极上，用于在-50 mV下安培测定过氧化氢。普鲁士蓝铜层在pH 7.4的条件下稳定2小时，保存时间为2个月。普鲁士的蓝铜修饰微电极用于开发基于氧化酶的乙醇和乳酸微生物传感器。通过与戊二醛交联固定乳酸和醇氧化酶。最高浓度为0.5 mM的可能的干扰化合物均未观察到干扰。乳酸微生物传感器的校准图在0.005至0.2 mM之间呈线性，检出限为0.0035 mM乳酸。酒精微生物传感器的线性动态范围在0.05至2 mM之间，检出限为0.02 mM乙醇。先进的微生物传感器用于分析腌黄瓜汁，酸奶和葡萄酒。

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