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首页> 外文期刊>Biomedical Chromatography: An International Journal Devoted to Research in Chromatographic Methodologies and Their Applications in the Biosciences >A sensitive fluorescence reagent, 2-(2-(7H-dibenzo(a,g)carbazol-7-yl)-ethoxy)ethyl chloroformate, for amino acids determination in Saussurea involucrate and Artemisia capillaris Thunb using high-performance liquid chromatography with fluorescence detection and identification with mass spectroscopy/electrospray ionization source.
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A sensitive fluorescence reagent, 2-(2-(7H-dibenzo(a,g)carbazol-7-yl)-ethoxy)ethyl chloroformate, for amino acids determination in Saussurea involucrate and Artemisia capillaris Thunb using high-performance liquid chromatography with fluorescence detection and identification with mass spectroscopy/electrospray ionization source.

机译：灵敏的荧光试剂2-（2-（2-（7H-二苯并（a，g）咔唑-7-基）-乙氧基）乙基）氯甲酸乙酯，用于雪莲和青蒿中氨基酸的测定，采用高效液相色谱法和荧光质谱/电喷雾电离源进行检测和鉴定。

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Recent researches shows that amino acids (AA) are not only cell signaling molecules but are also regulators of gene expression and the protein phosphorylation cascade. More precise analysis of AA composition is reckoned to be one of the most important applications in the biomedical and pharmaceutical fields. In this paper, we develop a sample, sensitive and mild method using 2-[2-(7H-dibenzo[a,g]carbazol-7-yl)-ethoxy]ethyl chloroformate (DBCEC) as A labeling reagent for AA determination by high-performance liquid chromatography (HPLC) with fluorescence detection (FLD) and identification with mass spectroscopy. The maximum excitation and emission wavelengths for DBCEC-AA derivatives were 300 and 395 nm, respectively. This method, in conjunction with a gradient elution, offered a baseline resolution of 20 AA on a reversed-phase Hypersil BDS C(18) column. LC separation for the derivatized AA showed good reproducibility, and all AA were found to give excellent linear responses with correlation coefficients > 0.9993. The calculated detection limits with a 25.0 fmol injection of each AA (at a signal-to-noise ratio of 3:1) ranged from 2.62 to 22.6 fmol. This method was applied to determine the AA composition in Saussurea involucrate and Artemisia capillaris Thunb. Meanwhile, this method exhibits a powerful potential for trace analysis of AA from biomedicine, foodstuff and other complex samples. Copyright (c) 2010 John Wiley & Sons, Ltd.
机译：最近的研究表明，氨基酸（AA）不仅是细胞信号分子，而且还是基因表达和蛋白质磷酸化级联的调节剂。氨基酸组成的更精确分析被认为是生物医学和制药领域最重要的应用之一。在本文中，我们开发了一种样品灵敏，温和的方法，使用2- [2-（7H-二苯并[a，g]咔唑-7-基）-乙氧基]乙基氯甲酸乙酯（DBCEC）作为A标记试剂，用于通过AA测定AA具有荧光检测（FLD）的高效液相色谱（HPLC）和质谱鉴定。 DBCEC-AA衍生物的最大激发和发射波长分别为300和395 nm。该方法与梯度洗脱相结合，在反相Hypersil BDS C（18）色谱柱上提供了20 AA的基线分离度。衍生化AA的LC分离具有良好的重现性，发现所有AA均具有出色的线性响应，相关系数> 0.9993。每次AA注入25.0 fmol（信噪比为3：1）时计算出的检测限范围为2.62至22.6 fmol。该方法用于测定雪莲和毛蒿蒿中的AA组成。同时，该方法具有对生物医学，食品和其他复杂样品中的氨基酸进行痕量分析的强大潜力。版权所有（c）2010 John Wiley＆Sons，Ltd.

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