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首页> 外文期刊>Biomedical Chromatography: An International Journal Devoted to Research in Chromatographic Methodologies and Their Applications in the Biosciences >Studies on target tissue distribution of ginsenosides and epimedium flavonoids in rats after intravenous administration of Jiweiling freeze-dried powder
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Studies on target tissue distribution of ginsenosides and epimedium flavonoids in rats after intravenous administration of Jiweiling freeze-dried powder

机译：吉味灵冻干粉静注大鼠体内人参皂甙和淫羊总黄酮靶组织分布的研究

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摘要

A simple and rapid liquid chromatography-mass spectrometry (LC-MS) method was developed and validated for analysis of ginsenoside Rb 1, Rb 2, Rc, Rd, Re, Rf, Rg 1, icariin and epimedin A, B, C in rat target tissues (spinal cord, brain, muscle and sciatic nerve) after intravenous administration of Jiweiling freeze-dried powder using genistein as an internal standard (IS). The tissue samples were treated by protein precipitation with methanol prior to HPLC and chromatographic separation was performed on a C 18 column utilizing a gradient elution program with acetonitrile and 0.1% formic acid aqueous. Electrospray ionization (ESI) source was employed and the 11 analytes and IS were detected by multiple reaction monitoring (MRM) scanning under the negative ionization mode. Higher sensitivity was achieved and the optimized mass transition ion-pairs (m/z) for quantitation were selected. The calibration curves were linear over the investigated concentration ranges with correlation coefficients higher than 0.995. The intra- and inter-day RSDs were all less than 10% with the relative error (RE) within ±9.3%. The mean extraction recoveries for all compounds were between 93.3 and 106%. The proposed method was successfully applied to investigate the target tissue distribution of the 11 compounds in rat after intravenous administration of Jiweiling freeze-dried powder.
机译：建立了一种简单快速的液相色谱-质谱（LC-MS）方法，并验证了该方法可用于分析人参皂苷Rb 1，Rb 2，Rc，Rd，Re，Rf，Rg 1，尿素和淫羊in苷A，B，C使用染料木黄酮作为内标（IS）静脉注射吉味灵冻干粉后，可将目标组织（脊髓，大脑，肌肉和坐骨神经）靶向。在进行HPLC之前，通过用甲醇进行蛋白质沉淀来处理组织样品，并在C 18柱上使用乙腈和0.1％甲酸水溶液的梯度洗脱程序进行色谱分离。使用电喷雾电离（ESI）源，并在负电离模式下通过多反应监测（MRM）扫描检测了11种分析物和IS。获得了更高的灵敏度，并选择了用于定量的优化质量转移离子对（m / z）。校准曲线在所研究的浓度范围内是线性的，相关系数高于0.995。日内和日间RSD均小于10％，相对误差（RE）在±9.3％以内。所有化合物的平均提取回收率在93.3％至106％之间。该方法成功地用于研究吉味灵冻干粉静脉内给药后11种化合物在大鼠体内的靶组织分布。

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