• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Annals of medicine >A novel screening method detects herpesviral DNA in the idiopathic pulmonary fibrosis lung
【24h】

A novel screening method detects herpesviral DNA in the idiopathic pulmonary fibrosis lung

机译：一种新的筛查方法，用于检测特发性肺纤维化肺中的疱疹病毒DNA

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background. Herpesviruses could contribute to the lung epithelial injury that initiates profibrotic responses in idiopathic pulmonary fibrosis (IPF). Methods. We identified herpesviral DNA from IPF and control lung tissue using a multiplex PCR-and microarray-based method. Active herpesviral infection was detected by standard methods, and inflammatory cell subtypes were identified with specific antibodies. Patients that underwent lung transplantation were monitored for signs of herpesviral infection. Results. A total of 11/12 IPF samples were positive for Epstein-Barr virus (EBV) and 10/12 for human herpesvirus 6B (HHV-6B) DNA. Control lung samples (n = 10) were negative for EBV DNA, whereas three samples were positive for HHV-6B. EBV-encoded RNA (EBER) was identified in nine IPF samples and localized mainly to lymphocytic aggregates. HHV-6B antigens were detected in mononuclear cells in IPF lung tissue. CD20+ B lymphocytic aggregates that were surrounded by CD3+ T cells were abundant in IPF lungs. CD23+ cells (activated B cells, EBV-transformed lymphoblasts, and dendritic cells) were observed in the aggregates. IPF patients had no signs of increased herpesviral activation after lung transplantation. Conclusions. Inflammatory cells are the main source of herpesviral DNA in the human IPF lung. Diagnostic tools should be actively used to elucidate whether herpesviral infection affects the pathogenesis, progression, and/or exacerbation of IPF.
机译：背景。疱疹病毒可能会导致肺上皮损伤，从而引发特发性肺纤维化（IPF）中的纤维化反应。方法。我们从IPF中鉴定出疱疹病毒DNA，并使用基于多重PCR和微阵列的方法控制肺组织。通过标准方法检测到活跃的疱疹病毒感染，并用特异性抗体鉴定了炎性细胞亚型。监测接受肺移植的患者疱疹病毒感染的迹象。结果。共有11/12 IPF样本的爱泼斯坦-巴尔病毒（EBV）阳性，而10/12的人类疱疹病毒6B（HHV-6B）DNA阳性。对照肺样本（n = 10）的EBV DNA阴性，而三份样本的HHV-6B阳性。在9个IPF样本中鉴定出EBV编码的RNA（EBER），并主要定位于淋巴细胞聚集体。在IPF肺组织的单核细胞中检测到HHV-6B抗原。 IPF肺中富含CD3 + T细胞的CD20 + B淋巴细胞聚集体。在聚集体中观察到CD23 +细胞（活化的B细胞，EBV转化的淋巴母细胞和树突状细胞）。 IPF患者在肺移植后没有疱疹病毒激活增加的迹象。结论炎性细胞是人类IPF肺中疱疹病毒DNA的主要来源。应积极使用诊断工具来阐明疱疹病毒感染是否会影响IPF的发病，进展和/或恶化。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号