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Application of nuclear magnetic resonance spectroscopy combined with principal component analysis in detecting inborn errors of metabolism using blood spots:a metabonomic approach

机译:核磁共振波谱结合主成分分析在利用血斑检测先天性代谢错误中的应用:一种代谢组学方法

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摘要

NMR spectra of extracted blood spots were used to investigate the possibility for the development of a new method for mass screening concerning the diagnosis of inborn errors of metabolism (IEM).Blood spots were collected on filter papers from normal,phenylketonuric (PKU) and maple syrup urine disease (MSUD) subjects and their Carr-Purcell-Meiboom-Gill (CPMG) ~1H NMR spectra were acquired.The spectra were reduced to a number of spectral descriptors and principal component analysis (PCA) was performed.The scores plot showed that PKU and MSUD samples were well discriminated from the main cluster of points.
机译:利用提取的血斑的NMR谱图研究开发一种用于诊断代谢先天性错误(IEM)的大规模筛查新方法的可能性。从正常纸,苯丙酮尿酸(PKU)和枫木滤纸上收集血斑采集糖浆尿病(MSUD)受试者及其Carr-Purcell-Meiboom-Gill(CPMG)〜1H NMR谱图,将谱图简化为许多谱图描述符,并进行主成分分析(PCA)。 PKU和MSUD样本与主要聚类点有很好的区别。

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