Techniques for Studying Protein Trafficking and Molecular Motors in Neurons

Feng Shanxi; Arnold Don B.

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Techniques for Studying Protein Trafficking and Molecular Motors in Neurons

机译：神经元蛋白贩运和分子马达的技术

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This review focused on techniques that facilitated the visualization of protein trafficking. In the mid-1990s the cloning of GFP allowed fluorescently tagged proteins to be expressed in cells and then visualized in real time. This advance allowed a glimpse, for the first time, of the complex system within cells for distributing proteins. It quickly became apparent, however, that time-lapse sequences of exogenously expressed GFP-labeled proteins can be difficult to interpret. Reasons for this include the relatively low signal that comes from moving proteins and high background rates from stationary proteins and other sources, as well as the difficulty of identifying the origins and destinations of specific vesicular carriers. In this review a range of techniques that have overcome these issues to varying degrees was reviewed and the insights into protein trafficking that they have enabled were discussed. Concentration will be on neurons, as they are highly polarized and, thus, their trafficking systems tend to be accessible for study. (C) 2016 Wiley Periodicals, Inc.

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来源
《Cytoskeleton》 |2016年第9期|共8页
作者
Feng Shanxi; Arnold Don B.;
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作者单位

Univ Southern Calif Dept Biol Sci Program Neurosci Los Angeles CA 90089 USA;

Univ Southern Calif Dept Biol Sci Program Neurosci Los Angeles CA 90089 USA;

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原文格式 PDF
正文语种 eng
中图分类细胞生物学;
关键词
protein trafficking; kinesin; myosin; vesicle; neuron;

机译：蛋白质贩运;Kinesin;肌球蛋白;囊泡;神经元;

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机译：运动神经元（SMN）蛋白与mRNA结合蛋白IMP1相互作用的存活动力学促进其向运动神经元轴突的运输。
6. High-efficiency transfection of cultured primary motor neurons to study protein localization, trafficking, and function [J] . Claudia Fallini, Gary J Bassell, Wilfried Rossoll Molecular neurodegeneration . 2010,第1期

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9. Cyclin-dependent kinase-5 regulates the polarized trafficking of dense-core vesicles in C. elegans motor neurons. [D] . Goodwin, Patricia Rayner. 2011

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10. Techniques for studying protein trafficking and molecular motors in neurons [O] . Shanxi Feng, Don B. Arnold -1

机译：研究神经元中蛋白质运输和分子运动的技术
11. High-efficiency transfection of cultured primary motor neurons to study protein localization, trafficking, and function [O] . Bassell Gary J, Fallini Claudia, Rossoll Wilfried 2010

机译：高效转染培养的原代运动神经元，研究蛋白质定位，运输和功能
12. Neuronal Functions Associated with Endo- and Exocytotic Events-cum-Molecular Trafficking May Be Cell Maturation-Dependent: Lessons Learned from Studies on Botulism. [R] . P. Ray P. Zhang R. Ray 2011

机译：与内吞和胞吐事件相关的神经元功能 - 暨 - 分子贩运可能是细胞成熟依赖性：从肉毒杆菌研究中吸取的教训。

Techniques for Studying Protein Trafficking and Molecular Motors in Neurons

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