...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biomedical Chromatography: An International Journal Devoted to Research in Chromatographic Methodologies and Their Applications in the Biosciences >Gradient elution mode for the troubleshooting of matrix effect on the determination of G004 in different tissues by LC-MS/MS
【24h】

Gradient elution mode for the troubleshooting of matrix effect on the determination of G004 in different tissues by LC-MS/MS

机译：通过LC-MS / MS梯度洗脱模式对基质效应对不同组织中G004的测定进行故障排除

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A steep gradient elution mode was applied to reduce the risk of matrix effect (ME) for the determination of G004, a novel sulfonylurea hypoglycemic drug, in a tissue distribution study by LC-MS/MS. The mass spectra of the total-ion-current chromatograms combined with the post-column infusion traces enabled the unseen' interfering species to be directly detected, and ensured that the chromatography conditions and sample preparation method were adequate to overcome the ME. According to this, a steep gradient elution mode was designed to overcome the intense ME from different tissues. The analysis was performed by monitoring the transitions m/z 558.1419.0 for G004 and m/z 489.3364.1 for glimepiride used as the internal standard. Calibration curves recovered over a range from 0.1 to 10000ng/mL for seven different tissues. Sex-related difference was found in the tissue distribution. The drug levels in the tissues of female rats were about two to three times higher than those in male counterparts. The highest level was observed in liver, then in kidney, heart, pancreas, lung and spleen, but no G004 was detected in brain. G004 was slowly eliminated from female rats compared with male rats. There was no long-term accumulation of G004 in male or female rat tissues. Copyright (c) 2014 John Wiley & Sons, Ltd.
机译：在通过LC-MS / MS进行的组织分布研究中，采用了陡峭的梯度洗脱模式来降低测定新的磺酰脲类降血糖药G004的基质效应（ME）风险。总离子流色谱图的质谱与柱后注入痕量相结合，可以直接检测到看不见的干扰物，并确保色谱条件和样品制备方法足以克服ME。据此，设计了一种陡峭的梯度洗脱模式来克服来自不同组织的强ME。通过监测G004的过渡m / z 558.1419.0和用作内标的格列美脲的m / z 489.3364.1进行分析。七种不同组织的校准曲线在0.1至10000ng / mL的范围内恢复。在组织分布中发现性别相关差异。雌性大鼠组织中的药物水平比雄性大鼠高约2至3倍。在肝脏中观察到最高水平，然后在肾脏，心脏，胰腺，肺和脾中观察到最高水平，但在脑中未检测到G004。与雄性大鼠相比，G004从雌性大鼠中被缓慢清除。在雄性或雌性大鼠组织中没有长期积累的G004。版权所有（c）2014 John Wiley＆Sons，Ltd.

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号