Establishment andin vitromorphogenesis of sapucaia explants (Lecythidaceae)

de Mello Tamyris; Goncalves Elzimar de Oliveira; Alexandre Rodrigo Sobreira; Schmildt Edilson Romais; Otoni Wagner Campos

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Establishment andin vitromorphogenesis of sapucaia explants (Lecythidaceae)

机译：Sapcaia外植体的紫素血管血管生殖植物（Lecythidiceae）

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摘要

Lecythis pisonisCambess, popularly known as sapucaia, has great economic and socio-environmental potential. The objective of this study was to evaluate the establishment andin vitromorphogenesis ofL. pisonisunder the effect of disinfecting agents, plant growth regulators, and thermal stress. The study was divided into three experiments: (i) development of the disinfection protocol by testing different concentrations and times of exposure to sodium hypochlorite (NaOCl) and different concentrations and methods of amoxicillin application, (ii)in vitrobudding induction by testing different concentrations of 6-benzylaminopurine (BAP) or kinetin (KIN) supplemented to Woody Plant Medium (WPM) and Murashige and Skoog (MS) culture media, and (iii)in vitroformation from plantlets by analyzing different concentrations of indole-3-butyric acid (IBA) with different exposure times to a thermal stress of 40 degrees C. The disinfection of stem segments was effective using 3% NaOCl and 3.0 g L(-1)amoxicillin solution. MS culture medium supplemented with 0.25 mg L(-1)BAP induced more shootsin vitro. One milligram per liter IBA promoted greater rootingin vitro, and it is not necessary for thermal stress tolerance.

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来源
《In Vitro Cellular and Development Biology. Plant: Journal of the Tissue Culture Association》 |2020年第6期|共12页
作者
de Mello Tamyris; Goncalves Elzimar de Oliveira; Alexandre Rodrigo Sobreira; Schmildt Edilson Romais; Otoni Wagner Campos;
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作者单位

Univ Fed Espirito Santo Programa Posgrad Ciencias Florestais Av Gov Lindemberg 316 BR-29550000 Jeronimo Monteiro ES Brazil;

Univ Fed Espirito Santo Programa Posgrad Ciencias Florestais Av Gov Lindemberg 316 BR-29550000 Jeronimo Monteiro ES Brazil;

Univ Fed Espirito Santo Programa Posgrad Ciencias Florestais Av Gov Lindemberg 316 BR-29550000 Jeronimo Monteiro ES Brazil;

Univ Fed Espirito Santo Ctr Univ Norte Espirito Santo Rodovia BR 101 Km 60 BR-29932540 Sao Mateus ES Brazil;

Univ Fed Vicosa Dept Biol Vegetal Ave Peter Henry Rolfs S-N Campus Univ BR-36570900 Vicosa MG Brazil;

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原文格式 PDF
正文语种 eng
中图分类植物学;细胞生物学;
关键词
Lecythis pisonis; in vitropropagation; Disinfectant agents; Growth regulators; Thermal stress;

机译：lecythis piso;在玻璃血管;消毒剂;生长调节剂;热应力;

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Establishment andin vitromorphogenesis of sapucaia explants (Lecythidaceae)

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