...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Analytical and bioanalytical chemistry >Facile preparation of organic-inorganic hybrid polymeric ionic liquid monolithic column with a one-pot process for protein separation in capillary electrochromatography
【24h】

Facile preparation of organic-inorganic hybrid polymeric ionic liquid monolithic column with a one-pot process for protein separation in capillary electrochromatography

机译：一锅法在毛细管电色谱中分离蛋白质的有机-无机杂化聚合物离子液体整体柱的快速制备

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

An organic-inorganic hybrid monolithic column based on 1-vinyl-3-dodecylimidazolium bromide (VC_(12)Im~+Br~?) has been prepared in a single step by combining radical copolymerization with a non-hydrolytic sol-gel (NHSG) process. The NHSG process was significantly short-ened to 6 h by using formic acid as catalyst. For comparison, we also prepared polymeric ionic liquid (PIL) monolithic columns by hydrolytic sol-gel and organic polymeric process, respectively. The resulting monolithic columns were characterized by Fourier transform infrared spectra, scanning electron microscopy, and Brunauer-Emmett-Teller. Under the capillary electrochromatography mode, these columns were applied to separate alkylbenzenes, anilines, and proteins, respectively. The results indicated that the NHSG-based hybrid PIL monolithic column exhibited the highest column efficiency among the three types of columns; organic solvent, commonly required by the traditional columns to achieve satisfactory separation efficiency for proteins, was absent in the NHSG-based hybrid PIL monolithic column because of the biocom-patibility of the VC_(12)Im~+Br~?, which was beneficial to analysis of protein containing samples. In order to demonstrate its application potential, the developed NHSGbased hybrid PIL monolithic column was also employed to separate egg white sample.
机译：通过将自由基共聚与非水解溶胶-凝胶（NHSG）结合使用，一步制备了一种基于1-乙烯基-3-十二烷基咪唑溴化物（VC_（12）Im〜+ Br〜？）的有机-无机杂化整体柱）过程。通过使用甲酸作为催化剂，NHSG工艺大大缩短至6小时。为了进行比较，我们还分别通过水解溶胶-凝胶法和有机聚合物法制备了聚合物离子液体（PIL）整体柱。通过傅立叶变换红外光谱，扫描电子显微镜和Brunauer-Emmett-Teller对所得的整体柱进行表征。在毛细管电色谱模式下，这些色谱柱分别用于分离烷基苯，苯胺和蛋白质。结果表明，在三种类型的色谱柱中，基于NHSG的混合PIL整体柱表现出最高的色谱柱效率。由于VC_（12）Im〜+ Br〜？的生物相容性，基于NHSG的混合PIL整体柱缺少传统色谱柱通常需要的有机溶剂才能实现令人满意的蛋白质分离效率。分析含蛋白质的样品。为了证明其应用潜力，还使用了开发的基于NHSG的混合PIL整体柱来分离蛋白样品。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号