...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Animal Production Science >Effect of elevated temperatures on bovine corpus luteum function: expression of heat-shock protein 70, cell viability and production of progesterone and prostaglandins by cultured luteal cells.
【24h】

Effect of elevated temperatures on bovine corpus luteum function: expression of heat-shock protein 70, cell viability and production of progesterone and prostaglandins by cultured luteal cells.

机译：高温对黄体功能的影响：热休克蛋白70的表达，细胞活力以及培养的黄体细胞产生黄体酮和前列腺素的能力。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Summer heat stress lowers fertility in cattle in hot environments by influencing oocyte quality, follicular activity and progesterone (P4) level in blood plasma. However, the mechanisms by which elevated temperature influences corpus luteum function remain unclear. Elevated temperature has generally been known to upregulate the gene expression of heat-shock protein (HSP) 70 in a variety of cell types. To clarify the direct effects of elevated temperature on bovine corpus luteum function, we examined the expressions of HSP70, cell viability and the production of P4 and prostaglandins (PGs) in luteal cells cultured at 37.5 degrees C (normal temperature in our culture system), 39.0 degrees C (moderately elevated temperature) or 41.0 degrees C (severely elevated temperature) for 12 or 24 h. HSP70 mRNA expression was increased by incubation at 39.0 degrees C for 12 h and at 41.0 degrees C for 12 and 24 h, whereas HSP70 protein expression was not significantly affected. The viability of luteal cells cultured for 24 h, measured by flow cytometry with propidium iodide staining, was not significantly affected by temperature. Interestingly, the production of P4 by cultured luteal cells was higher at 39.0 degrees C than at 37.5 degrees C after 12 and 24 h of incubation. The production of PGF2 alpha was higher at 39.0 degrees C and 41.0 degrees C than at 37.5 degrees C after 12 and 24 h of incubation. The production of PGE2 was higher at 41.0 degrees C than at 37.5 degrees C after 24 h of incubation. The overall results suggested that elevated temperature does not negatively affect luteal function, and that the low fertility observed during summer is not due to a direct effect of elevated temperature on luteal cells.
机译：夏季高温胁迫通过影响血浆中的卵母细胞质量，卵泡活性和孕酮（P4）水平，降低了炎热环境下牛的生育能力。但是，高温影响黄体功能的机制尚不清楚。通常已知升高温度会上调多种细胞类型中的热休克蛋白（HSP）70的基因表达。为了阐明高温对黄体功能的直接影响，我们研究了在37.5摄氏度（我们培养系统的正常温度）下培养的黄体细胞中HSP70的表达，细胞活力以及P4和前列腺素（PGs）的产生，在39.0摄氏度（中度高温）或41.0摄氏度（中度高温）下持续12或24小时。通过在39.0摄氏度，12小时和41.0摄氏度下孵育12和24小时，HSP70 mRNA的表达增加，而HSP70蛋白的表达没有受到明显影响。通过碘化丙啶染色的流式细胞仪测量，培养24小时的黄体细胞的活力不受温度的显着影响。有趣的是，培养12到24小时后，培养的黄体细胞在39.0摄氏度下产生的P4高于37.5摄氏度。孵育12和24小时后，在39.0摄氏度和41.0摄氏度下，PGF2α的产量高于37.5摄氏度。孵育24小时后，PGE2的产量在41.0摄氏度时高于37.5摄氏度。总体结果表明，高温不会对黄体功能产生负面影响，而且夏季观察到的低生育力并不是由于温度升高对黄体细胞的直接影响。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号