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首页> 外文期刊>Annals of Forest Science >Genetic variation, mating patterns and gene flow in a Pinus pinaster Aiton clonal seed orchard.
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Genetic variation, mating patterns and gene flow in a Pinus pinaster Aiton clonal seed orchard.

机译：樟子松无性系种子园的遗传变异，交配模式和基因流。

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摘要

Relatedness among parents, variation in clonal fertility and background pollination deviate the realized genetic gain and the genetic diversity of open pollinated seed orchard from expectation, in particular in wind pollinated species such as Pinus pinaster. This work investigates the genetic variation, the mating system and the pollen contamination in a P. pinaster clonal seed orchard (CSO), by screening the 60 clones from the CSO and the seeds collected from 21 mother-trees with three nuclear microsatellites. The selected clonal seed orchard is located in Escaroupim National Forest in Portugal. The expected diversity was similar, but the observed heterozygosity decreased 20% in the progenies compared with the parental trees. The outcrossing rate was 90.1%, the biparental inbreeding 21.7% computed through a multilocus approach, and the observed selfing 3.9%. The observed gene flow from outside the CSO was 52.4%. From the results we concluded that the observed gene flow and the biparental inbreeding were high, and care should be taken in the implementation and management of future CSO, in particular clones should be checked for relatedness and the ramet number could be directly proportional to their breeding value.
机译：父母之间的亲缘关系，克隆繁殖力的变化和背景授粉的情况，使开放授粉种子园的已实现遗传增益和遗传多样性与预期相背离，特别是在风授粉物种（如松树）中。这项工作调查了遗传变异，交配系统和花粉中的花粉污染。 Pinaster克隆种子园（CSO），方法是通过筛选来自CSO的60个克隆和从具有21个母树的3个核微卫星中收集的种子。选定的无性种果园位于葡萄牙的Escaroupim国家森林。预期的多样性相似，但是与亲本树相比，后代中观察到的杂合度降低了20％。异源杂交率为90.1％，通过多位点方法计算的双亲近交率为21.7％，观察到的自交率为3.9％。从CSO外部观察到的基因流量为52.4％。从结果中我们得出结论，观察到的基因流和双亲近亲繁殖都很高，并且在将来的CSO的实施和管理中应格外小心，特别是应检查克隆的相关性，并且分株数可能与它们的繁殖成正比值。

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