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首页> 外文期刊>Biochimica et biophysica acta: BBA: International journal of biochemistry, biophysics and molecular biololgy. Proteins and Proteomics >Comparison of functional properties of mammalian DNA polymerase lambda and DNA polymerase beta in reactions of DNA synthesis related to DNA repair
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Comparison of functional properties of mammalian DNA polymerase lambda and DNA polymerase beta in reactions of DNA synthesis related to DNA repair

机译：比较哺乳动物DNA聚合酶lambda和DNA聚合酶beta在与DNA修复有关的DNA合成反应中的功能特性

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摘要

DNA polymerase lambda (Pol lambda) is a novel enzyme of the family X of DNA polymerases. Pol lambda has some properties in common with DNA polymerase beta (Pol beta). The substrate properties of Pol lambda were compared to Pol beta, using DNAs mimicking short-patch (SP) and long-patch (LP) base excision repair (BER) intermediates as well as recessed template primers. In the present work, the influence of several BER proteins such as flap-endonuclease-1 (FEN1), PCNA, and apurinic/apyrimidinic endonuclease-1 (APEI) on the activity of Pol lambda was investigated. Pol lambda is unable to catalyze strand displacement synthesis using nicked DNA, although this enzyme efficiently incorporates a dNMP into a one-nucleotide gap. FENI and PCNA stimulate the strand displacement activity of Pol lambda. FENI processes nicked DNA, thus removing a barrier to Pol lambda DNA synthesis. It results in a one-nucleotide gapped DNA molecule that is a favorite substrate of Pol lambda. Photocrosslinking and functional assay show that Pol lambda is less efficient than Pol beta in binding to nicked DNA. APEI has no influence on the strand displacement activity of Pol lambda though it stimulates strand displacement synthesis catalyzed with Pol beta. It is suggested that Pol lambda plays a role in the SP BER rather than contributes to the LP BER pathway. (c) 2005 Elsevier B.V. All rights reserved.
机译：DNA聚合酶lambda（Pol lambda）是DNA聚合酶X家族的新型酶。 Pol lambda具有与DNA聚合酶beta（Pol beta）相同的某些属性。使用模仿短补丁（SP）和长补丁（LP）碱基切除修复（BER）中间体的DNA以及凹入的模板引物，将Pol lambda的底物性质与Pol beta进行了比较。在目前的工作中，研究了几种BER蛋白，例如皮瓣内切核酸酶1（FEN1），PCNA和紫质/嘧啶内切核酸酶1（APEI）对λλ活性的影响。 Pol lambda无法使用带切口的DNA催化链置换合成，尽管该酶有效地将dNMP整合到一个核苷酸的缺口中。 FENI和PCNA刺激Polλ的链置换活性。 FENI处理有切口的DNA，从而消除了PolλDNA合成的障碍。它产生了一个单核苷酸缺口的DNA分子，它是Polλ的最爱底物。光交联和功能测定表明，Pol lambda与带切口的DNA结合的效率低于Polβ。 APEI虽然会刺激Polβ催化的链置换合成，但对Polλ的链置换活性没有影响。建议Pol lambda在SP BER中起作用，而不是在LP BER途径中起作用。 （c）2005 Elsevier B.V.保留所有权利。

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