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首页> 外文期刊>Analytical methods >Mean centering of ratio spectra for colorimetric determination of morphine and codeine in pharmaceuticals and biological samples using melamine modified gold nanoparticles
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Mean centering of ratio spectra for colorimetric determination of morphine and codeine in pharmaceuticals and biological samples using melamine modified gold nanoparticles

机译：使用三聚氰胺修饰的金纳米粒子比色法测定药物和生物样品中吗啡和可待因的比率谱的平均居中

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摘要

Driven by the need to detect narcotics, a new and simple colorimetric assay is presented for the sensitive and visual detection of morphine (MOR) and codeine (COD) using melamine modified gold nanoparticles (MA-AuNPs). Hydrogen-bonding interactions between melamine and the drugs induce aggregation of the AuNPs with a consequent color change from wine red to blue, which can be monitored by a UV-vis spectrophotometer or even the naked eye. Furthermore, we demonstrated the practicality of using mean centering of ratio spectra (MCRS) to remove the contribution of the reagent blank and obtain the net analyte signal in nanoparticle-based colorimetric assays, an aspect which appears to have received no attention to date. This procedure gives more accurate results than the conventional approach using the absorption ratio at maximum wavelength of the aggregated state to the dispersion state (A_(agg)/A_(dis)). Comparison of the proposed method with the usual method reveals that MCRS slightly improves the figures of merit of the colorimetric assays. The systems exhibited a linear range from 0.07 to 3 μM for MOR and from 0.03 to 0.8 μM for COD using MCRS. Detection limits (3σ/m) of 17 and 9 nM were achieved for MOR and COD, respectively, which are much lower than the therapeutic plasma/serum concentrations and cutoff levels for opiates in urine samples set by the National Institute on Drug Abuse (NIDA). Furthermore, we demonstrate the application of the approach in biological fluids and pharmaceuticals, which suggests that the present assay could satisfy the requirements of clinical toxicology and forensic cases.
机译：出于对麻醉品检测的需求，提出了一种新的简单的比色测定法，用于使用三聚氰胺修饰的金纳米颗粒（MA-AuNPs）进行灵敏和可视化的吗啡（MOR）和可待因（COD）检测。三聚氰胺与药物之间的氢键相互作用可诱导AuNP聚集，从而使颜色从酒红色变为蓝色，可通过紫外可见分光光度计甚至肉眼对其进行监测。此外，我们证明了在基于纳米粒子的比色测定中使用比率光谱的平均居中（MCRS）来消除试剂空白的影响并获得净分析物信号的实用性，这一方面迄今为止尚未引起人们的注意。与使用聚集状态最大波长到分散状态的最大吸收比（A_（agg）/ A_（dis））的常规方法相比，此过程可提供更准确的结果。所提出的方法与常规方法的比较表明，MCRS稍微提高了比色测定的品质因数。使用MCRS，系统对MOR的线性范围为0.07至3μM，对于COD的线性范围为0.03至0.8μM。 MOR和COD的检出限（3σ/ m）分别达到17和9 nM，远低于美国国家药物滥用研究所（NIDA）设定的尿样中阿片类药物的治疗性血浆/血清浓度和截止水平）。此外，我们证明了该方法在生物液体和药物中的应用，这表明本测定法可以满足临床毒理学和法医案件的要求。

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