...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Parasitology >Development of an immunochromatographic device to detect antibodies for rapid diagnosis of human angiostrongyliasis
【24h】

Development of an immunochromatographic device to detect antibodies for rapid diagnosis of human angiostrongyliasis

机译：探测免疫色谱术装置，以检测人类血管生长细胞快速诊断的抗体

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Cerebral angiostrongyliasis is a central nervous system disease caused by Angiostrongylus cantonensis and can produce eosinophilic meningitis or meningoencephalitis in humans. Sero-immunological techniques, such as the enzyme-linked immunosorbent assay (ELISA) and immunoblotting, are most commonly used for the diagnosis of human angiostrongyliasis. However, diagnosis in remote areas remains problematic because sophisticated equipment and specialized skills are required. To overcome, we have developed the immunochromatographic test (ICT) kit for rapid serological diagnosis of angiostrongyliasis through the detection of anti-A. cantonensis-specific antibodies in human serum. A recombinant A, cantonensis galec-tin-2 (rAcGal2) from young adult female worms was used as an antigen for the ICT kit development. Diagnostic values were evaluated and compared using the ELISA. The sensitivity, specificity and positive and negative predictive values of the ICT kit were 87.0, 96.5, 94.6 and 91.4%, respectively, and those of the ELISA were 91.0, 97.2, 95.8 and 94.0%, respectively. The concordance of the ICT kit was 93.9%. We, thus, determined that the ICT kit is sensitive and specific and provides reliable diagnostic results. It is rapid and simple to perform and can be utilized for both point-of-care diagnosis in the bedside laboratory and epidemiological surveys in endemic regions where access to diagnostic equipment is limited.
机译：脑血管圆线虫病是一种由广州管圆线虫引起的中枢神经系统疾病，可在人体内引起嗜酸性脑膜炎或脑膜脑炎。血清免疫学技术，如酶联免疫吸附试验（ELISA）和免疫印迹法，最常用于人类血管圆线虫病的诊断。然而，偏远地区的诊断仍然存在问题，因为需要复杂的设备和专业技能。为了克服这一问题，我们开发了免疫层析检测（ICT）试剂盒，通过检测人血清中的抗广州管圆线虫特异性抗体，对广州管圆线虫病进行快速血清学诊断。从年轻成年雌性蠕虫中提取的重组广州管圆线虫galec-tin-2（rAcGal2）被用作ICT试剂盒开发的抗原。使用ELISA评估和比较诊断值。ICT试剂盒的敏感性、特异性、阳性和阴性预测值分别为87.0%、96.5%、94.6%和91.4%，ELISA试剂盒的敏感性、特异性、阳性和阴性预测值分别为91.0%、97.2%、95.8%和94.0%。ICT试剂盒的一致性为93.9%。因此，我们确定ICT试剂盒敏感、特异，并提供可靠的诊断结果。它操作简单快速，可用于床边实验室的护理点诊断，以及对诊断设备使用有限的流行地区的流行病学调查。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号