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首页> 外文期刊>Animal Reproduction Science >Comparison of the BullMate (TM) sperm quality analyzer with conventional means of assessing the semen quality and breeding soundness of beef bulls
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Comparison of the BullMate (TM) sperm quality analyzer with conventional means of assessing the semen quality and breeding soundness of beef bulls

机译：BullMate（TM）精子质量分析仪与评估牛公牛精液质量和繁殖健全性的常规方法的比较

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摘要

An instrument called the Optibreed(TM), BullMate(TM) sperm quality analyzer (SQA) contains a densitometer for determining sperm cell concentration and an optical sensor to evaluate light deflections caused by sperm movement. Analysis of light deflections enables the generation of a value called the sperm quality index (SQI). The SQI represents the quality of a semen sample defined by sperm motility, concentration, viability and morphology. The SQA was compared to conventional, microscopic techniques for determining percent motile sperm and sperm concentration in bull semen samples and evaluated for its ability to classify bulls as satisfactory or unsatisfactory potential breeders. Semen samples were collected from 105 mature beef bulls by electroejaculation (day 1) and from 51 of the same bulls by internal artificial vagina (IAV) on day 2. SQI values were arranged into 20 categories in increments of 50 units from 0 to 1000 units. Percent motile sperm and sperm concentration values from both methods were significantly positively correlated (P < 0.000) with respective r values of 0.82 and 0.80. A calculation of kappa to evaluate the differences in percent motile sperm generated by each system yielded a value of 0.20 and 0.54 for unweighted and weighted determinations, respectively. The intraclass correlation coefficient used to evaluate the reliability of sperm cell concentration determinations was 0.62 (P < 0.05). SQI values generated on days I and 2 ranged from 0-994 to 0-906, respectively. Bulls were categorized as satisfactory or unsatisfactory potential breeders in all categories. The most appropriate SQI for determining whether a bull was a satisfactory or unsatisfactory potential breeder was 500 with respective sensitivity, specificity, positive predictive values (PPV) and negative predictive values (NPV) of 81, 65, 90.79 and 44.83%. In this experiment the BullMate(TM) SQA was not a reliable substitute for conventional semen analysis and was not useful for determining bull breeding soundness.
机译：一种称为OptibreedTM，BullMateTM精子质量分析仪（SQA）的仪器包含用于确定精子细胞浓度的光密度计和用于评估由精子运动引起的光偏转的光学传感器。通过分析光的偏转，可以生成称为精子质量指数（SQI）的值。 SQI代表由精子活力，浓度，生存力和形态定义的精液样品的质量。将SQA与传统的显微技术进行比较，以确定公牛精液样本中的活动精子百分比和精子浓度，并评估其将公牛分类为令人满意或不满意的潜在育种者的能力。在第2天，通过电射精（第1天）从105头成熟的公牛采集精液样本，在第2天，通过内部人工阴道（IAV）从相同的公牛51头精液样本中采集。SQI值分为20类，以50单位从0到1000单位递增。两种方法的运动精子百分比和精子浓度值均显着正相关（P <0.000），其r值分别为0.82和0.80。计算每个系统产生的运动精子百分比差异的卡伯值计算得出，未加权和加权测定的值分别为0.20和0.54。用于评估精子细胞浓度测定的可靠性的类内相关系数为0.62（P <0.05）。第I天和第2天生成的SQI值分别从0-994到0-906。公牛在所有类别中均被归类为令人满意或不令人满意的潜在育种者。用于确定公牛是令人满意的还是不令人满意的潜在育种者的最合适的SQI为500，其敏感性，特异性，阳性预测值（PPV）和阴性预测值（NPV）分别为81、65、90.79和44.83％。在该实验中，BullMate TM SQA不是常规精液分析的可靠替代品，并且对确定公牛繁殖的稳健性没有用。

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