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首页> 外文期刊>Animal Reproduction Science >Sperm cryopreservation of sex-reversed seven-band grouper, Epinephelus septemfasciatus
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Sperm cryopreservation of sex-reversed seven-band grouper, Epinephelus septemfasciatus

机译：逆向性七带石斑鱼（Epinephelus septemfasciatus）的精子冷冻保存

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Seven-band grouper Epinephelus septemfasciatus is a protogynous hermaphrodite. The male individuals' number is more less than the female one. Thus, it is necessary for artificial reproduction and crossbreeding to research the sperm cryopreservation of sex-reversed seven-band grouper. In present study, the spermatozoa of sex-reversed immature fish were frozen using the different cryopreserving solutions for cryopreservation. The several factors that may affect the freezing survival rate of seven-band grouper spermatozoa such as the spermatozoa diluent, the concentration and composition of cryoprotective agent have been studied. The results showed that ES 1-3 (60 g/L glucose +10 g/L NaCl + 0.5 g/L NaHCO3) was significantly better as a diluent compared with MPRS, TS-2 and other series of diluent ES1. The further experiment revealed that the optimal cryoprotectants were 10% dimethyl sulfoxide (DMSO) or 10% 1,2-propylene glycol (PG) with the post-thaw sperm motility was 76.67 +/- 0.00% and 75.00 +/- 5.00%, respectively. In addition, salinity of seawater is an important motility stimulator because that the highest motility of 96.00 +/- 1.73% was obtained at salinity 30 parts per thousand. In crossbreeding test with fresh unfertilized eggs of kelp grouper Epinephelus moara, the ratio of fertilization and hatchability had no significant differences between the cryopreserved sperm and fresh sperm of seven-band grouper. It is suggested that the frozen sperm of seven-band grouper could be applied in artificial reproduction and crossbreeding
机译：七带石斑鱼Epinephelus septemfasciatus是雌雄同体的雌雄同体。男性人数比女性人数少。因此，对于人工繁殖和杂交育种，研究逆向性七波段石斑鱼的精子冷冻保存是必要的。在本研究中，使用不同的冷冻保存液冷冻保存反转性未成熟鱼的精子。研究了影响七波段石斑鱼冷冻存活率的几个因素，例如精子稀释剂，冷冻保护剂的浓度和组成。结果表明，与MPRS，TS-2和其他系列稀释剂ES1相比，ES 1-3（60 g / L葡萄糖+10 g / L NaCl + 0.5 g / L NaHCO3）作为稀释剂要好得多。进一步的实验表明，最佳的冷冻保护剂是10％的二甲基亚砜（DMSO）或10％的1,2-丙二醇（PG），解冻后的精子活力为76.67 +/- 0.00％和75.00 +/- 5.00％，分别。另外，海水的盐度是重要的动力刺激剂，因为在千分之30的盐度下，最高的动力为96.00 +/- 1.73％。在海带石斑鱼石斑鱼新鲜未受精卵的杂交试验中，冷冻保存的精子和七波段石斑鱼的新鲜精子的受精率和孵化率没有显着差异。建议将七带石斑鱼冷冻精子用于人工繁殖和杂交。

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