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首页> 外文期刊>Primates >Stability of chimpanzee (Pan troglodytes) urinary reproductive hormones during long-term preservation on filter paper
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Stability of chimpanzee (Pan troglodytes) urinary reproductive hormones during long-term preservation on filter paper

机译：在滤纸上长期保存期间黑猩猩（PAN Troglodytes）尿生殖激素的稳定性

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Urine contains multiple water-soluble hormones, which are valuable non-invasive biomarkers for the monitoring of reproductive status and health. An effective method for drying urine on filter paper was previously developed to preserve wildlife urine samples where electrical equipment was not available for this; however, the stability of samples preserved in this way remains to be verified. Here, we developed and validated a method to elute multiple water-soluble reproductive hormones from filter paper that had been stored for an extended period of time. Aliquots of urine from chimpanzees were adsorbed on filter papers, air dried and stored for 1 year at room temperature. Estrone-3-conjugate (E1C), pregnanediol-3-glucuronide (PdG), estriol-3-glucuronide (E(3)G), and chorionic gonadotropin (CG) were eluted into deionized water from the filter papers and measured using enzyme immunoassays (EIAs). The mean recoveries of E1C, PdG, and creatinine from filter papers stored for 1 year were 69.5%, 128.7%, and 83.8%, respectively. The profiles of E1C and PdG from preserved filter papers significantly correlated with those derived from a direct analysis of the frozen urine of menstruating chimpanzees. We detected E(3)G and CG from 1-year-old filter papers for urine collected during early pregnancy, but the recovery of E(3)G was low and CG profiles did not correlate with those of the original frozen urine samples. The method proposed here for the elution and measurement of reproductive hormones in urine preserved for a long period of time on filter paper provides a practical and simple way to monitor the reproductive status of chimpanzees. We propose that this method can also be utilized in field studies of other wild nonhuman primates.
机译：尿液中含有多种水溶性激素，是监测生殖状态和健康的有价值的非侵入性生物标志物。在没有电气设备的情况下，先前开发了一种在滤纸上干燥尿液的有效方法，以保存野生动物尿液样本；然而，以这种方式保存的样品的稳定性仍有待验证。在这里，我们开发并验证了一种方法，可以从长期储存的滤纸中洗脱多种水溶性生殖激素。将黑猩猩的尿液等份吸附在滤纸上，风干，并在室温下保存1年。将雌酮-3-结合物（E1C）、孕二醇-3-葡萄糖醛酸（PdG）、雌三醇-3-葡萄糖醛酸（E（3）G）和绒毛膜促性腺激素（CG）从滤纸中洗脱到去离子水中，并使用酶免疫分析法（EIAs）进行测量。贮存1年的滤纸中E1C、PdG和肌酐的平均回收率分别为69.5%、128.7%和83.8%。从保存过的滤纸中提取的E1C和PdG图谱与直接分析经期黑猩猩冰冻尿液得到的图谱显著相关。我们从1年前的滤纸中检测了妊娠早期收集的尿液中的E（3）G和CG，但E（3）G的回收率较低，CG曲线与原始冷冻尿液样本的曲线不相关。本文提出的在滤纸上长期保存的尿液中生殖激素的洗脱和测量方法，为监测黑猩猩的生殖状况提供了一种实用而简单的方法。我们认为这种方法也可以用于其他野生非人灵长类动物的野外研究。

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