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首页> 外文期刊>Platelets >An increased expression profile of Th9/IL-9 correlated with Th17/IL-17 in patients with immune thrombocytopenia
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An increased expression profile of Th9/IL-9 correlated with Th17/IL-17 in patients with immune thrombocytopenia

机译：TH9 / IL-9的表达谱与免疫血小板减少症患者的TH17 / IL-17相关

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摘要

Immune thrombocytopenia (ITP) is a heterogeneous autoimmune disease, characterized by dysregulation of cellular immunity. Th9 cells were recently identified as a new subtype of Th cells, characterized by preferential production of IL-9. Given the pleiotropic function of IL-9, Th9 cells are demonstrated to be involved in various autoimmune diseases. However, whether Th9 cells are involved in the pathogenesis of ITP remains unclear. In this study, 49 active ITP patients, 39 ITP with remission and 20 healthy controls were included. Peripheral blood mononuclear cells (PBMCs) were isolated from ITP and controls for measuring Th9 and Th17 cells by flow cytometry. Meanwhile, RNA was isolated from PBMCs for the measurement of the mRNA level of PU.1, IRF4, BATF, and RORt by quantitative real-time PCR. Plasma levels of IL-9 and IL-17 were detected by ELISA. Our results showed that higher expressions of Th9, IL-9, and associated transcription factors (PU.1, IRF4, and BATF) were found in active ITP patients and restored to the normal level (except IL-9) in patients in remission. Meanwhile, Th9 cells and the IL-9 plasma level were positively correlated with Th17 cells and the IL-17 level in ITP patients, respectively. Moreover, a positive correlation of IRF4 or BATF with RORt was found. In conclusion, an aberrant expression profile of Th9/IL-9 was associated with pathogenesis of ITP possibly through cooperatively working with Th17/IL-17 and therapeutically targeting Th9/IL-9 might be a novel approach in the treatment of ITP.
机译：免疫性血小板减少症（ITP）是一种异质性自身免疫性疾病，以细胞免疫失调为特征。Th9细胞最近被鉴定为Th细胞的一个新亚型，其特征是优先产生IL-9。鉴于IL-9的多效性功能，Th9细胞被证明与各种自身免疫疾病有关。然而，Th9细胞是否参与ITP的发病机制尚不清楚。在这项研究中，包括49名活动期ITP患者、39名缓解期ITP患者和20名健康对照者。从ITP和对照组中分离外周血单个核细胞（PBMC），用流式细胞术检测Th9和Th17细胞。同时，从外周血单个核细胞中提取RNA，用于测定PU的mRNA水平。1、实时定量PCR检测IRF4、BATF和RORt。ELISA法检测血浆IL-9和IL-17水平。我们的研究结果表明，在活动期ITP患者中发现Th9、IL-9和相关转录因子（PU.1、IRF4和BATF）的高表达，在缓解期患者中恢复到正常水平（IL-9除外）。同时，ITP患者的Th9细胞和IL-9血浆水平分别与Th17细胞和IL-17水平呈正相关。此外，发现IRF4或BATF与RORt呈正相关。综上所述，Th9/IL-9的异常表达谱可能通过与Th17/IL-17协同工作与ITP的发病机制相关，以Th9/IL-9为靶点的治疗可能是治疗ITP的一种新方法。

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  • 来源
    《Platelets》 |2017年第3期|共8页
  • 作者

    Qiao Jianlin; Li Xiaoqian; Wu Yulu; Wu Xiaoqing; Zhu Feng; Liu Na; Qi Kunming; Cheng Hai; Li Depeng; Sun Haiying; Zeng Lingyu; Xu Kailin;

  • 作者单位

    Xuzhou Med Univ Blood Dis Inst 84 West Huaihai Rd Xuzhou 221002 Peoples R China;

    Xuzhou Med Univ Blood Dis Inst 84 West Huaihai Rd Xuzhou 221002 Peoples R China;

    Xuzhou Med Univ Blood Dis Inst 84 West Huaihai Rd Xuzhou 221002 Peoples R China;

    Xuzhou Med Univ Blood Dis Inst 84 West Huaihai Rd Xuzhou 221002 Peoples R China;

    Xuzhou Med Univ Dept Hematol Affiliated Hosp 99 West Huaihai Rd Xuzhou 221002 Jiangsu Peoples;

    Tengzhou Cent Peoples Hosp Dept Hematol Tengzhou Peoples R China;

    Xuzhou Med Univ Dept Hematol Affiliated Hosp 99 West Huaihai Rd Xuzhou 221002 Jiangsu Peoples;

    Xuzhou Med Univ Dept Hematol Affiliated Hosp 99 West Huaihai Rd Xuzhou 221002 Jiangsu Peoples;

    Xuzhou Med Univ Dept Hematol Affiliated Hosp 99 West Huaihai Rd Xuzhou 221002 Jiangsu Peoples;

    Xuzhou Med Univ Dept Hematol Affiliated Hosp 99 West Huaihai Rd Xuzhou 221002 Jiangsu Peoples;

    Xuzhou Med Univ Blood Dis Inst 84 West Huaihai Rd Xuzhou 221002 Peoples R China;

    Xuzhou Med Univ Blood Dis Inst 84 West Huaihai Rd Xuzhou 221002 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 基础医学;
  • 关键词

    IL-9; IL-17; immune thrombocytopenia; Th9; Th17;

    机译：IL-9;IL-17;免疫血小板减少;TH9;TH17;

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