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Harmonizing light transmission aggregometry in the Netherlands by implementation of the SSC-ISTH guideline

机译：通过实施SSC-ISTH指南，协调荷兰光传输聚合物

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Light transmission aggregometry (LTA) is considered the gold standard method for evaluation of platelet function. However, there are a lot of variation in protocols (pre-analytical procedures and agonist concentrations) and results. The aim of our study was to establish a national LTA protocol, to investigate the effect of standardization and to define national reference values for LTA. The SSC guideline was used as base for a national procedure. Almost all recommendations of the SSC were followed e.g. no adjustment of PRP, citrate concentration of 109 mM, 21 needle gauge, fasting, resting time for whole blood and PRP, centrifugation time, speed and agonists concentrations. LTA of healthy volunteers was measured in a total of 16 hospitals with 5 hospitals before and after standardization. Results of more than 120 healthy volunteers (maximum aggregation %) were collected, with participating laboratories using 4 different analyzers with different reagents. Use of low agonist concentrations showed high variation before and after standardization, with the exception of collagen. For most high agonist concentrations (ADP, collagen, ristocetin, epinephrine and arachidonic acid) variability in healthy subjects decreased after standardization. We can conclude that a standardized Dutch protocol for LTA, based on the SSC guideline, does not result in smaller variability in healthy volunteers for all agonist concentrations.
机译：光透射聚集仪（LTA）被认为是评价血小板功能的金标准方法。然而，在方案（分析前程序和激动剂浓度）和结果上有很多差异。我们研究的目的是建立国家LTA协议，调查标准化的效果，并确定LTA的国家参考值。SSC指南被用作国家程序的基础。几乎所有SSC的建议都得到了遵守，例如没有调整PRP、109 mM柠檬酸盐浓度、21针规、禁食、全血和PRP的静息时间、离心时间、速度和激动剂浓度。在标准化前后，共有16家医院和5家医院对健康志愿者的LTA进行了测量。收集了120多名健康志愿者（最大聚集率%）的结果，参与实验室使用4种不同的分析仪和不同的试剂。除胶原蛋白外，低浓度激动剂的使用在标准化前后表现出很大的差异。对于大多数高浓度激动剂（ADP、胶原蛋白、瑞斯托西丁、肾上腺素和花生四烯酸），标准化后健康受试者的变异性降低。我们可以得出结论，基于SSC指南的标准化荷兰LTA方案不会导致健康志愿者在所有激动剂浓度下的较小可变性。

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