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首页> 外文期刊>Urolithiasis. >Urinary MCP-1 EuroHMGB1 increased in calcium nephrolithiasis patients and the influence of hypercalciuria on the production of the two cytokines
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Urinary MCP-1 EuroHMGB1 increased in calcium nephrolithiasis patients and the influence of hypercalciuria on the production of the two cytokines

机译：尿MCP-1 EurohmgB1增加肾肾血症患者和高钙血管对两种细胞因子的影响的影响

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摘要

The study aims to observe the urinary excretion of monocyte chemoattractant-1 (MCP-1) and high-mobility group box 1 (HMGB1) in patients with calcium nephrolithiasis and to determine the influence of hypercalciuria on the production of the two cytokines. 81 cases of patients with calcium nephrolithiasis (group CN) and 30 healthy controls (group C) were involved in this study. To observe the influence of urinary calcium on the excretion of those cytokines, the patients were subdivided according to their 24-h urinary calcium level: >= 4 mg/kg/day (group H) and < 4 mg/kg/day (group N). MCP-1 and HMGB1 in urina sanguinis were determined for all subjects. In addition, in vitro study was done to determine the production of the two cytokines and index of apoptosis and oxidative injuries in human kidney epithelial cells (HK-2) exposed to three high levels of calcium. Data showed that both urinary MCP-1 and HMGB1 in group CN were higher than that of group C. When the patients were subdivided, comparisons among the three groups showed that both MCP-1 and HMGB1 in group H and group N were higher than group C, but there was no significant statistical difference between the two stone groups. In vitro study, the apoptosis rate of cells, the lactate dehydrogenase activities, the hydrogen peroxide, and 8-isoprostane concentrations in the medium all increased in accordance with the increased concentration of calcium supplemented. Compared with the control, mRNA expressions of MCP-1 and HMGB1 in cells and the protein concentrations of the two cytokines in the medium of calcium-supplemented groups increased significantly. Results showed that urinary MCP-1 and HMGB1 increased in calcium nephrolithiasis patients and hypercalciuria might affect the identical pathways (through the reactive oxygen species) with other factors in stimulating the production of MCP-1 and HMGB1 in vivo.
机译：本研究旨在观察钙肾结石患者尿中单核细胞趋化因子-1（MCP-1）和高迁移率族蛋白B1（HMGB1）的排泄情况，并确定高钙尿对这两种细胞因子产生的影响。81例钙肾结石患者（CN组）和30例健康对照者（C组）参与本研究。为了观察尿钙对这些细胞因子排泄的影响，根据24小时尿钙水平对患者进行细分：>=4 mg/kg/天（h组）和<4 mg/kg/天（N组）。检测所有受试者血尿液中的MCP-1和HMGB1。此外，还进行了体外研究，以确定暴露于三种高水平钙的人类肾上皮细胞（HK-2）中两种细胞因子的产生以及凋亡和氧化损伤指数。数据显示，CN组的尿MCP-1和HMGB1均高于C组。当对患者进行细分时，三组之间的比较显示，H组和N组的MCP-1和HMGB1均高于C组，但两组之间无显著统计学差异。在体外研究中，细胞凋亡率、乳酸脱氢酶活性、过氧化氢和培养基中的8-异前列腺素浓度均随钙浓度的增加而增加。与对照组相比，补钙组细胞中MCP-1和HMGB1的mRNA表达以及两种细胞因子在培养基中的蛋白质浓度显著增加。结果表明，钙肾结石患者尿MCP-1和HMGB1升高，高钙尿症可能通过活性氧影响与其他因素相同的途径，刺激体内MCP-1和HMGB1的产生。

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  • 来源
    《Urolithiasis.》 |2017年第2期|共17页
  • 作者

    Wang Yang; Sun Chun; Li Chengyang; Deng Yaoliang; Zeng Guohua; Tao Zhiwei; Wang Xiang; Guan Xiaofeng; Zhao Yutong;

  • 作者单位

    Guangxi Med Univ Dept Urol Affiliated Hosp 1 Nanning 530021 Peoples R China;

    Guangxi Med Univ Dept Urol Affiliated Hosp 1 Nanning 530021 Peoples R China;

    Guangxi Med Univ Dept Urol Affiliated Hosp 1 Nanning 530021 Peoples R China;

    Guangxi Med Univ Dept Urol Affiliated Hosp 1 Nanning 530021 Peoples R China;

    Guangzhou Med Univ Dept Urol Affiliated Hosp 1 Guangzhou Guangdong Peoples R China;

    Guangxi Med Univ Dept Urol Affiliated Hosp 1 Nanning 530021 Peoples R China;

    Guangxi Med Univ Dept Urol Affiliated Hosp 1 Nanning 530021 Peoples R China;

    Guangxi Med Univ Dept Urol Affiliated Hosp 1 Nanning 530021 Peoples R China;

    Guangxi Med Univ Dept Urol Affiliated Hosp 1 Nanning 530021 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 泌尿科学（泌尿生殖系疾病）;
  • 关键词

    Hypercalciuria; Inflammation; MCP-1; HMGB1; Oxidative stress;

    机译：hypercalciuria;炎症;MCP-1;HMGB1;氧化应激;

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