Multi-transcription factor reporter mice delineate early precursors to the ILC and LTi lineages

Kasal Darshan N.; Bendelac Albert

首页> 外文期刊>The British journal of psychiatry : >Multi-transcription factor reporter mice delineate early precursors to the ILC and LTi lineages

【24h】

Multi-transcription factor reporter mice delineate early precursors to the ILC and LTi lineages

机译：多转录因子报告小鼠将早期前体描绘给ILC和Lti谱系

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Transcription factor (TF) reporter mice have proved integral to the characterization of murine innate lymphoid cell (ILC) development and function. Here, we implemented a CRISPR/Cas9-generated combinatorial reporter approach for the simultaneous resolution of several key TFs throughout ILC development in both the fetal liver and adult bone marrow. We demonstrate that the Tcf7-expressing early innate lymphoid precursor (EILP) and the common helper ILC precursor (CHILP) both contain a heterogeneous mixture of specified ILC and lymphoid tissue inducer (LTi) precursors with restricted lineage potential rather than a shared precursor. Moreover, the earliest specified precursor to the LTi lineage was identified upstream of these populations, before Tcf7 expression. These findings match dynamic changes in chromatin accessibility associated with the expression of key TFs (i.e., GATA3 and ROR gamma(t)), highlighting the distinct origins of ILC and LTi lineages at the epigenetic and functional levels, and provide a revised map for ILC development.

机译：转录因子（TF）报告小鼠已被证明是小鼠固有淋巴细胞（ILC）发育和功能表征的组成部分。在这里，我们实施了一种CRISPR/Cas9生成的组合报告方法，用于同时解析胎儿肝脏和成人骨髓中ILC发育过程中的几个关键TF。我们证明，表达Tcf7的早期先天性淋巴前体（EILP）和常见辅助性ILC前体（CHILP）均含有特定ILC和淋巴组织诱导物（LTi）前体的异质性混合物，具有有限的谱系潜能，而不是共同的前体。此外，在Tcf7表达之前，在这些群体的上游发现了LTi谱系最早的特定前体。这些发现与关键TFs（即GATA3和ROR伽马（t））表达相关的染色质可及性的动态变化相匹配，突出了ILC和LTi谱系在表观遗传学和功能水平上的不同起源，并为ILC的发展提供了一个修订图。

著录项

来源
《The British journal of psychiatry :》 |2021年第2期|共21页
作者
Kasal Darshan N.; Bendelac Albert;
展开▼
作者单位

Univ Chicago Comm Immunol Chicago IL 60637 USA;

Univ Chicago Comm Immunol Chicago IL 60637 USA;

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类精神病学;
关键词

相似文献

外文文献
中文文献
专利

1. A report on Lineage switch at relapse of CD19 CAR-T therapy for Philadelphia chromosome-positive B-precursor acute lymphoblastic leukemia [J] . Lan-Zhu Li, Jian-Yong Li, Si-Xuan Qian, 中华医学杂志（英文版） . 2020,第016期
2. Expression and regulation of versican in neural precursor cells and their lineages [J] . Wen-li GU, Sai-li FU, Yan-xia WANG, 中国药理学报：英文版 . 2007,第010期
3. Express=on of nerve growth factor precursor, mature nerve growth factor and their receptors during cerebral ischemia-reperfusion injury [J] . Guoqian He, Jian Guo, Jiachuan Duan, 中国神经再生研究：英文版 . 2011,第022期
4. Expression of nerve growth factor precursor, mature nerve growth factor and their receptors during cerebral ischemia-reperfusion injury [J] . Guoqian He, Jian Guo, Jiachuan Duan, 中国神经再生研究（英文版） . 2011,第022期
5. Sgn1, a basic helix-loop-helix transcription factor delineates the salivary gland duct cell lineage in mice [J] . Yoshida S, Ohbo K, Takakura A, Developmental biology . 2001,第2期

机译：Sgn1，一种基本的螺旋-环-螺旋转录因子描述了小鼠唾液腺导管细胞谱系
6. A reporter mouse model for in vivo tracing and in vitro molecular studies of melanocytic lineage cells and their diseases A reporter mouse model for in vivo tracing and in vitro molecular studies of melanocytic lineage cells and their diseases A reporter mouse model for in vivo tracing and in vitro molecular studies of melanocytic lineage cells and their diseases [J] . Lina Dagnino, Valerie Leclerc, Melissa Crawford Biology Open . 2017,第8期

机译：用于黑色素细胞谱系细胞及其疾病的体内追踪和体外分子研究的报告基因小鼠模型用于黑色素细胞谱系细胞及其疾病的体内追踪和体外分子研究的报告基因小鼠模型黑素细胞系细胞及其疾病的体外分子研究
7. A reporter mouse model for in vivo tracing and in vitro molecular studies of melanocytic lineage cells and their diseases A reporter mouse model for in vivo tracing and in vitro molecular studies of melanocytic lineage cells and their diseases A reporter mouse model for in vivo tracing and in vitro molecular studies of melanocytic lineage cells and their diseases [J] . Lina Dagnino, Valerie Leclerc, Melissa Crawford Biology Open . 2017,第8期

机译：用于黑色素细胞谱系细胞及其疾病的体内追踪和体外分子研究的报告基因小鼠模型用于黑色素细胞谱系细胞及其疾病的体内追踪和体外分子研究的报告基因小鼠模型黑素细胞系细胞及其疾病的体外分子研究
8. Non-invasive imaging of prostate cancer progression in nude mice using iRFP gene reporter [C] . Banghe Zhu, Grace Wu, Holly Robinson, Conference on photonic therapeutics and diagnostics IX . 2013

机译：使用iRFP基因报告子对裸鼠前列腺癌进展进行非侵入性成像
9. A genetic reporter/selection system for optimization of lineage specific embryonic stem cell differentiation. [D] . Handy, Cyrus Z. 2005

机译：用于优化谱系特异性胚胎干细胞分化的遗传报告基因/选择系统。
10. Fbxo2VHC mouse and embryonic stem cell reporter lines delineate in vitro-generated inner ear sensory epithelia cells and enable otic lineage selection and Cre-recombination [O] . Byron H. Hartman, Robert Böscke, Daniel C. Ellwanger, -1

机译：Fbxo2VHC小鼠和胚胎干细胞报告员系描绘了体外产生的内耳感觉上皮细胞并能进行耳道谱系选择和Cre重组
11. Sgn1, a Basic Helix-Loop-Helix Transcription Factor Delineates the Salivary Gland Duct Cell Lineage in Mice [O] . Yoshida Shosei, Ohbo Kazuyuki, Takakura Ayumi, 2001

机译：Sgn1，基本的螺旋环螺旋转录因子描述小鼠唾液腺导管细胞谱系。
12. Effects of Hematopoietic Lineage and Precursor Age on CML Disease Progression [R] . Dorshkind, K. 2007

机译：造血谱系和前体年龄对CmL疾病进展的影响

Multi-transcription factor reporter mice delineate early precursors to the ILC and LTi lineages

摘要

著录项

相似文献

相关主题

期刊订阅