...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The European Journal of Neuroscience >Construction of a 3D brain extracellular matrix model to study the interaction between microglia and T cells in co-culture
【24h】

Construction of a 3D brain extracellular matrix model to study the interaction between microglia and T cells in co-culture

机译：3D脑细胞外基质模型的构建研究了共培养中微胶质细胞和T细胞的相互作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Neurodegenerative disorders are characterised by the activation of brain-resident microglia cells and by the infiltration of peripheral T cells. However, their interplay in disease has not been clarified yet. It is difficult to investigate complex cellular dynamics in living animals, and simple two-dimensional (2D) cell culture models do not resemble the soft 3D structure of brain tissue. Therefore, we developed a biomi-metic 3D in vitro culture system for co-cultivation of microglia and T cells. As the activation and/or migration of immune cells in the brain might be affected by components of the extracellular matrix, defined 3D fibrillar collagen I-based matrices were constructed and modified with hyaluronan and/or chondroitin sulphate, resembling aspects of brain extracellular matrix. Murine microglia and spleen-derived T cells were cultured alone or in co-culture on the constructed matrices. Microglia exhibited in vivo-like morphology and T cells showed enhanced survival when co-cultured with microglia or to a minor degree in the presence of glia-conditioned medium. The open and porous fibrillar structure of the matrix allowed for cell invasion and direct cell-cell interaction, with stronger invasion of T cells. Both cell types showed no dependence on the matrix modifications. Microglia could be activated on the matrices by lipopolysaccharide resulting in interleukin-6 and tumour necrosis factor-α secretion. The findings herein indicate that biomimetic 3D matrices allow for co-cultivation and activation of primary microglia and T cells and provide useful tools to study their interaction in vitro.
机译：神经退行性疾病的特点是脑内驻留的小胶质细胞激活和外周T细胞浸润。然而，它们在疾病中的相互作用尚未阐明。在活体动物中研究复杂的细胞动力学是困难的，简单的二维（2D）细胞培养模型与脑组织的软3D结构不相似。因此，我们开发了一种仿生3D体外培养系统，用于小胶质细胞和T细胞的联合培养。由于脑内免疫细胞的激活和/或迁移可能受到细胞外基质成分的影响，因此构建并用透明质酸和/或硫酸软骨素修饰了定义的3D纤维I型胶原基质，类似于脑细胞外基质的各个方面。小鼠小胶质细胞和脾源性T细胞单独培养或在构建的基质上共同培养。当小胶质细胞与小胶质细胞共培养时，或在有胶质细胞条件培养基存在的情况下，小胶质细胞表现出类似体内的形态，T细胞显示出增强的存活率。基质的开放多孔纤维结构允许细胞入侵和直接的细胞间相互作用，T细胞的入侵更强。两种细胞类型均不依赖于基质修饰。脂多糖可激活基质上的小胶质细胞，从而分泌白细胞介素-6和肿瘤坏死因子-α。本文的研究结果表明，仿生3D基质允许原代小胶质细胞和T细胞的共同培养和激活，并为研究它们在体外的相互作用提供了有用的工具。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号