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首页> 外文期刊>The Journal of Nutritional Biochemistry >Heat treatment of galangin and kaempferol inhibits their benefits to improve barrier function in rat intestinal epithelial cells
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Heat treatment of galangin and kaempferol inhibits their benefits to improve barrier function in rat intestinal epithelial cells

机译：Galangin和Kaempferol的热处理抑制了它们在大鼠肠上皮细胞中改善屏障功能的益处

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摘要

Flavonols are bioactive substances in plant foods. In this study, two flavonols galangin and kaempferol were heated at 100 degrees C for 30 min prior to assessing their effects on barrier function of rat intestinal epithelial (IEC-6) cells. Both heated and unheated flavonols (2.5-20 mu mol/L dosages) were nontoxic to the cells up to 48 h post-treatment, and could promote cell viability values to 102.2-141.2% of control. By treatment with 5 mu mol/L flavonols for 24 and 48 h, the treated cells time-dependently showed better improved physical and biological barrier functions than the control cells without any flavonol treatment, including higher transepithelial electrical resistance and antibacterial effect but reduced paracellular permeability and bacterial translocation. The results from real-time PCR and western-blot assays indicated that the cells treated with heated and unheated flavonols of 5 mu mol/L dosage had up-regulated mRNA (1.13-1.81 folds) and protein (1.15-5.11 folds) expression for zonula occluden-1, occludin, and claudin-1 that are vital to the tight junctions of the cells. Moreover, protein expression of RhoA and ROCK were down-regulated into 0.41-0.98 and 0.40 -0.92 folds, respectively, demonstrating a Rho inactivation that led to enhanced cell barrier integrity via the RhoA/ROCK pathway. Overall, galangin was more active than kaempferol to perform three biofunctions like improving cell barrier function, up-regulating tight junctions protein expression, and down-regulating RhoA/ROCK expression. Moreover, the heated flavonols were less effective than the unheated counterparts to perform these biofunctions. It is concluded that this heat treatment of galangin and kaempferol could inhibit their benefits to improve barrier function of IEC-6 cells. (C) 2020 Elsevier Inc. All rights reserved.
机译：黄酮醇是植物性食品中的生物活性物质。在本研究中，两种黄酮醇高良姜素和山奈酚在100℃下加热30分钟，然后评估它们对大鼠肠上皮（IEC-6）细胞屏障功能的影响。加热和未加热的黄酮醇（2.5-20μmol/L剂量）在处理后48小时内对细胞无毒，并可将细胞活力值提高至对照的102.2-141.2%。经5μmol/L黄酮醇处理24和48小时后，与未经任何黄酮醇处理的对照细胞相比，经时间依赖性处理的细胞显示出更好的物理和生物屏障功能，包括更高的跨上皮电阻和抗菌效果，但降低了细胞旁通透性和细菌移位。实时PCR和western blot分析的结果表明，用加热和未加热的5μmol/L剂量的黄酮醇处理的细胞对细胞紧密连接至关重要的闭塞带-1、闭塞素和克劳丁-1的mRNA（1.13-1.81倍）和蛋白质（1.15-5.11倍）表达上调。此外，RhoA和ROCK的蛋白表达分别下调至0.41-0.98和0.40-0.92倍，表明Rho失活通过RhoA/ROCK途径增强了细胞屏障的完整性。总的来说，高良姜素在改善细胞屏障功能、上调紧密连接蛋白表达和下调RhoA/ROCK表达等三种生物功能方面比山奈酚更活跃。此外，加热的黄酮醇在执行这些生物功能方面不如未加热的黄酮醇有效。结论：高良姜素和山奈酚的这种热处理可以抑制它们改善IEC-6细胞屏障功能的益处。（C） 2020爱思唯尔公司版权所有。

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