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首页> 外文期刊>The journals of gerontology.Series A. Biological sciences and medical sciences >Skeletal Muscle Mitochondrial Adaptations to Maximal Strength Training in Older Adults
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Skeletal Muscle Mitochondrial Adaptations to Maximal Strength Training in Older Adults

机译：骨骼肌线粒体适应老年人最大力量训练

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Maximal strength training (MST) results in robust improvements in skeletal muscle force production, efficiency, and mass. However, the effects of MST on muscle mitochondria are still unknown. Accordingly, the purpose of this study was to examine, from the molecular level to whole-muscle, mitochondrial adaptations induced by 8 weeks of knee-extension MST in the quadriceps of 10 older adults using immunoblotting, spectrophotometry, high-resolution respirometry in permeabilized muscle fibers, in vivo P-31 magnetic resonance spectroscopy (P-31-MRS), and gas exchange. As anticipated, MST resulted in an increased isometric knee-extensor force from 133 +/- 36 to 147 +/- 49 Nm (p < .05) and quadriceps muscle volume from 1,410 +/- 103 to 1,555 +/- 455 cm(3) (p < .05). Mitochondrial complex (I-V) protein abundance and citrate synthase activity were not significantly altered by MST. Assessed ex vivo, maximal ADP-stimulated respiration (state 3(CI+CII), PRE: 23 +/- 6 and POST: 14 +/- 5 rho M.mg(-1).s(-1), p < .05), was decreased by MST, predominantly, as a result of a decline in complex I-linked respiration (p < .05). Additionally, state 3 free-fatty acid linked respiration was decreased following MST (PRE: 19 +/- 5 and POST: 14 +/- 3 rho M.mg(-1).s(-1), p < .05). Assessed in vivo, MST slowed the PCr recovery time constant (PRE: 49 +/- 13 and POST: 57 +/- 16 seconds, p < .05) and lowered, by similar to 20% (p = .055), the quadriceps peak rate of oxidative ATP synthesis, but did not significantly alter the oxidation of lipid. Although these, likely qualitative, mitochondrial adaptations are potentially negative in terms of skeletal muscle energetic capacity, they need to be considered in light of the many improvements in muscle function that MST affords older adults.
机译：最大力量训练（MST）能显著改善骨骼肌的力量产生、效率和质量。然而，MST对肌肉线粒体的影响尚不清楚。因此，本研究的目的是通过免疫印迹法、分光光度法、渗透性肌纤维的高分辨率呼吸测定法、活体P-31磁共振波谱（P-31-MRS）和气体交换法，从分子水平到整个肌肉，检测10名老年人的股四头肌在伸膝8周MST诱导下的线粒体适应。正如预期的那样，MST导致等长膝伸肌力从133+/-36增加到147+/-49 Nm（p<0.05），股四头肌体积从1410+/-103增加到1555+/-455 cm（3）（p<0.05）。线粒体复合物（I-V）蛋白丰度和柠檬酸合成酶活性未受MST显著改变。体外评估最大ADP刺激呼吸（状态3（CI+CII），前：23+/-6，后：14+/-5 rho M.mg（-1）。s（-1），p<0.05），MST降低，主要是由于复杂的I-连锁呼吸减少（p<0.05）。此外，MST后状态3游离脂肪酸相关呼吸降低（前：19+/-5和后：14+/-3 rho M.mg（-1）。s（-1），p<0.05）。在体内评估，MST减慢了PCr恢复时间常数（前：49+/-13秒和后：57+/-16秒，p<0.05），并降低了股四头肌氧化ATP合成的峰值速率，与20%（p=0.055）相似，但没有显著改变脂质的氧化。虽然这些可能是定性的线粒体适应在骨骼肌能量能力方面可能是负面的，但需要考虑到MST为老年人提供的许多肌肉功能改善。

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