...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Annals of Botany >Developmental changes in spatial distribution of in vivo fluorescence and epidermal UV absorbance over Quercus petraea leaves.
【24h】

Developmental changes in spatial distribution of in vivo fluorescence and epidermal UV absorbance over Quercus petraea leaves.

机译：栎栎叶片上体内荧光和表皮紫外线吸收的空间分布的发育变化。

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Epidermal phenolic compounds (mainly flavonoids) constitute a vital screen that protects the leaf from damage by natural ultraviolet (UV) radiation. The effectiveness of epidermal UV-screening depends on leaf anatomy, the content of UV-screening compounds and their spatial uniformity over the leaf area. To investigate in vivo the spatial pattern of the epidermal UV-screen during leaf development, a fluorescence imaging method was developed to map the epidermal UV-absorbance at a microscopic scale. This study was done on oak (Quercus petraea) leaves that were used as a model of woody dicotyledonous leaves. METHODS: The leaf development of 2-year-old trees, grown outdoors, was monitored, at a macroscopic scale, by in vivo measurements of chlorophyll content per unit area and epidermal UV-absorbance using two optical leaf-clip meters. The distribution of pigments within leaves was assessed in vivo spectroscopically. The microscopic images of UV-induced fluorescence and UV-absorbance acquired in vivo during leaf development were interpreted from spectral characteristics of leaves. KEY RESULTS: At a macroscopic scale, epidermal UV-absorbance was high on the upper leaf side during leaf development, while it increased on the lower leaf side during leaf expansion and reached the adaxial value at maturity. At a microscopic scale, in immature leaves, for both leaf sides, the spatial distribution of epidermal UV-absorbance was heterogeneous, with a pattern depending on the flavonoid content of vacuoles in developing epidermal cells. At maturity, epidermal UV-absorbance was uniform. CONCLUSIONS: The spatial pattern of epidermal UV-screen over the area of oak leaves is related to leaf anatomy during development. In vivo spectroscopy and fluorescence imaging of the leaf surface showed the distribution of pigments within the leaf and hence can provide a tool to monitor optically the leaf development in nature.
机译：表皮酚类化合物（主要是类黄酮）构成了重要的屏障，可保护叶片免受自然紫外线（UV）辐射的损害。表皮紫外线屏蔽的有效性取决于叶片的解剖结构，紫外线屏蔽化合物的含量及其在整个叶区域的空间均匀性。为了在体内研究叶片发育过程中表皮紫外线屏蔽的空间模式，开发了一种荧光成像方法以微观尺度绘制表皮紫外线吸收率。这项研究是在用作木质双子叶叶片模型的橡树叶上进行的。方法：通过使用两个光学叶夹仪对每单位面积的叶绿素含量和表皮紫外线吸收率进行体内测量，从宏观角度监测生长在室外的2岁树的叶子发育。体内光谱法评估叶内色素的分布。从叶片的光谱特性解释了叶片发育过程中在体内获得的紫外线诱导的荧光和紫外线吸收的显微图像。主要结果：在宏观尺度上，叶片发育过程中上叶侧的表皮紫外线吸收率很高，而在叶片膨胀过程中下叶侧的表皮紫外线吸收率增加，并在成熟时达到近轴值。在微观尺度上，在未成熟的叶子中，对于叶子的两面，表皮紫外线吸收的空间分布是不均匀的，其模式取决于发育中的表皮细胞中液泡的类黄酮含量。成熟时，表皮的紫外线吸收是均匀的。结论：橡树叶上的表皮紫外线屏幕的空间格局与发育过程中的叶片解剖结构有关。叶片表面的体内光谱和荧光成像显示了色素在叶片中的分布，因此可以提供一种光学监测自然界中叶片发育的工具。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号