Validation of the TaqMan Influenza A Detection Kit and a rapid automated total nucleic acid extraction method to detect influenza A virus in nasopharyngeal specimens.

Bolotin S; De Lima C; Choi KW; Lombos E; Burton L; Mazzulli T; Drews SJ

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Validation of the TaqMan Influenza A Detection Kit and a rapid automated total nucleic acid extraction method to detect influenza A virus in nasopharyngeal specimens.

机译：验证TaqMan甲型流感检测试剂盒和快速自动全核酸提取方法以检测鼻咽标本中的甲型流感病毒。

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This study describes the validation of the TaqMan Influenza A Detection Kit v2.0 combined with an automated nucleic acid extraction method. The limit of detection of this assay was determined by probit regression (95% confidence interval) to be 2 influenza A/PR/8/34 (H1N1) virus particles per microlitre. One hundred and eleven specimens previously tested using the Seeplex RV assay and viral culture methods were tested using the TaqMan Influenza A Detection Kit. Compared to the aggregate gold-standard, the sensitivity and specificity of the TaqMan Influenza A Detection Kit were 100% (35/35) and 97% (74/76), respectively. Because of its accuracy, quick turn-around-time and lyophilized bead form, the TaqMan Influenza A Detection Kit, combined with the NucliSense easyMAG automated extraction method, constitutes a reliable protocol for influenza A diagnosis.

机译：这项研究描述了结合自动核酸提取方法的TaqMan甲型流感检测试剂盒v2.0的验证。通过概率回归（95％置信区间）确定此测定的检测极限为每微升2个流感A / PR / 8/34（H1N1）病毒颗粒。使用TaqMan甲型流感检测试剂盒对先前使用Seeplex RV分析和病毒培养方法测试的111个标本进行了测试。与总的金标准相比，TaqMan甲型流感检测试剂盒的灵敏度和特异性分别为100％（35/35）和97％（74/76）。由于其准确性，快速的周转时间和冻干的小珠形式，TaqMan甲型流感检测试剂盒与NucliSense easyMAG自动提取方法相结合，构成了诊断甲型流感的可靠方案。

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《Annals of Clinical and Laboratory Science: Official Journal of the Association of Clinical Scientists》 |2009年第2期|共5页
作者
Bolotin S; De Lima C; Choi KW; Lombos E; Burton L; Mazzulli T; Drews SJ;
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正文语种 eng
中图分类基础医学;
关键词
Automation; DNA; Viral; Disease Outbreaks; Humans; Influenza A virus; Influenza; Human; Nasopharynx; RNA; Viral; Regression Analysis; Reproducibility of Results; Reverse Transcriptase Polymerase Chain Reaction; Seasons; Sensitivity and Specificity; Specimen Handling; *自动化; DNA; 病毒; 疾病暴发流行; 鼻咽; RNA; 病毒; 回归分析; 可重复性; 结果; 逆转录聚合酶链反应; 季节; 易感性与特异性; 标本制备;

机译：Automation;DNA;Viral;Disease Outbreaks;Humans;Influenza A virus;Influenza;Human;Nasopharynx;RNA;Viral;Regression Analysis;Reproducibility of Results;Reverse Transcriptase Polymerase Chain Reaction;Seasons;Sensitivity and Specificity;Specimen Handling;*自动化;DNA;病毒;疾病暴发流行;鼻咽;RNA;病毒;回归分析;可重复性;结果;逆转录聚合酶链反应;季节;易感性与特异性;标本制备;

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1. Validation of the TaqMan Influenza A Detection Kit and a rapid automated total nucleic acid extraction method to detect influenza A virus in nasopharyngeal specimens. [J] . Bolotin S, De Lima C, Choi KW, Annals of Clinical and Laboratory Science: Official Journal of the Association of Clinical Scientists . 2009,第2期

机译：验证TaqMan甲型流感检测试剂盒和快速自动全核酸提取方法以检测鼻咽标本中的甲型流感病毒。
2. Evaluation of a rapid isothermal nucleic acid amplification kit, Alere (TM) i Influenza A&B, for the detection of avian influenza viruses [J] . Bazarragchaa Enkhbold, Okamatsu Masatoshi, Ulaankhuu Ankhanbaatar, Journal of Virological Methods . 2019,第期

机译：评估快速等温核酸扩增试剂盒，Salere（TM）I流感A＆amp; B，用于检测禽流感病毒
3. Influenza and respiratory syncytial virus detection in clinical specimens without nucleic acid extraction using FOCUS direct disc assay is substantially equivalent to the traditional methods and the FOCUS nucleic acid extraction-dependent RT-PCR assay [J] . SelvarajuS.B., TierneyD., LeberA.L., Diagnostic microbiology and infectious disease . 2014,第3期

机译：不使用FOCUS直接圆盘测定法提取核酸的临床标本中的流感和呼吸道合胞病毒检测基本上等同于传统方法以及FOCUS依赖核酸提取的RT-PCR测定法
4. Evaluation of Flu DETECT antigen test kit using three different sampling methods during natural infections of influenza A virus in swine [C] . Joseph P. Yaros, Brett K. OBrien, Joel L. Nerem, American Association of Swine Veterinarians Annual Meeting . 2015

机译：利用三种不同抽样方法评价流感检测抗原试剂盒在猪流感病毒自然感染期间
5. A Portable Impedance Biosensing System based on a Laptop with LabVIEW for Rapid Detection of Avian Influenza Virus [D] . Wang, Yixiang. 2014

机译：基于LabVIEW的便携式笔记本电脑阻抗生物传感系统，用于禽流感病毒的快速检测
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Validation of the TaqMan Influenza A Detection Kit and a rapid automated total nucleic acid extraction method to detect influenza A virus in nasopharyngeal specimens.

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