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首页> 外文期刊>Annals of Botany >Flow cytometric analysis of pollen grains collected from individual bees provides information about pollen load composition and foraging behaviour.
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Flow cytometric analysis of pollen grains collected from individual bees provides information about pollen load composition and foraging behaviour.

机译：从单个蜜蜂采集的花粉粒的流式细胞仪分析可提供有关花粉负载组成和觅食行为的信息。

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Background and Aims: Understanding the species composition of pollen on pollinators has applications in agriculture, conservation and evolutionary biology. Current identification methods, including morphological analysis, cannot always discriminate taxa at the species level. Recent advances in flow cytometry techniques for pollen grains allow rapid testing of large numbers of pollen grains for DNA content, potentially providing improved species resolution. Methods: A test was made as to whether pollen loads from single bees (honey-bees and bumble-bees) could be classified into types based on DNA content, and whether good estimates of proportions of different types could be made. An examination was also made of how readily DNA content can be used to identify specific pollen species. Key Results: The method allowed DNA contents to be quickly found for between 250 and 9391 pollen grains (750-28 173 nuclei) from individual honey-bees and between 81 and 11 512 pollen grains (243-34 537 nuclei) for bumble-bees. It was possible to identify a minimum number of pollen species on each bee and to assign proportions of each pollen type (based on DNA content) present. Conclusions: The information provided by this technique is promising but is affected by the complexity of the pollination environment (i.e. number of flowering species present and extent of overlap in DNA content). Nevertheless, it provides a new tool for examining pollinator behaviour and between-species or cytotype pollen transfer, particularly when used in combination with other morphological, chemical or genetic techniques.
机译：背景与目的：了解授粉媒介上花粉的种类组成在农业，保护和进化生物学中都有应用。当前的识别方法（包括形态分析）无法始终在物种级别上区分分类单元。用于花粉粒的流式细胞术技术的最新进展允许快速测试大量花粉粒的DNA含量，从而可能提供改善的物种分辨率。方法：测试是否可以根据DNA含量将单个蜜蜂（蜜蜂和大黄蜂）的花粉负荷分类为不同类型，以及是否可以很好地估计不同类型的比例。还检查了DNA含量如何容易地用于鉴定特定的花粉种类。关键结果：该方法可以快速发现单个蜜蜂的250至9391个花粉粒（750-28 173核）和大黄蜂的81至11512个花粉粒（243-34 537核）的DNA含量。 。可以确定每只蜜蜂上最少的花粉种类，并可以分配每种花粉类型的比例（基于DNA含量）。结论：该技术提供的信息是有希望的，但受授粉环境的复杂性影响（即存在的开花物种数量和DNA含量重叠的程度）。但是，它提供了一种检查传粉媒介行为以及种间或细胞型花粉转移的新工具，特别是与其他形态，化学或遗传技术结合使用时。

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