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首页> 外文期刊>Zygote >Seminal characterization of the Amazonian fire-eye tetra Moenkhausia oligolepis (Gunther, 1864)
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Seminal characterization of the Amazonian fire-eye tetra Moenkhausia oligolepis (Gunther, 1864)

机译：亚马逊灭火Tetra Moenkhausia Oligolepis（Gunther，1864）的开创性。

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The seminal characteristics of Moenkhausia oligolepis are described. Three males were induced with a single dose of carp pituitary. Semen was collected 6 h after induction, and diluted in dibasic sodium phosphate extender solution. For motility analysis, 1 mu l of diluted semen was added to 10 mu l of distilled water to achieve gamete activation. The average duration of total motility was 76.67 s; while the average sperm motility rate at intervals of 15 s was 95.3, 85.3, 59.6, 31.7, 13.0, 4.6 and 1.2%. To determine sperm concentration in samples, 0.5 mu l of semen was diluted with 500 mu l of glutaraldehyde. An aliquot of 10 mu l of this dilution was utilized for cell counting. An average count of 4.97 x 10(9) +/- 3.46 sperm/ml was obtained. Morphological analyses were performed using eosin-nigrosine dye; 20.33% of the sperm were observed to be dead. Live sperm, comprising the other 79.67%, had an average length of approximately 30 mu m, with a head diameter of 4.488 +/- 0.7 mu m; and a flagella plus mid-piece length of 26.071 +/- 12.4 mu m. Of those sperm, 69% had a normal morphology, while 31% had primary and secondary abnormalities. The observed abnormality rate did not have a detrimental effect on artificial fertilization potential for the species. The description of the seminal characteristics of a species is one of the most important sets of information required for artificial reproduction of fish in captivity. It also contributes significantly to the total biological knowledge of the studied species.
机译：本文描述了寡叶莫恩豪斯菌的精液特征。用单剂量鲤鱼脑垂体诱导三只雄性。诱导6小时后收集精液，并在二元酸磷酸钠稀释液中稀释。为了进行运动性分析，将1μl稀释精液添加到10μl蒸馏水中以实现配子激活。总运动时间平均为76.67s；而15s的平均精子活率分别为95.3%、85.3%、59.6%、31.7%、13.0%、4.6%和1.2%。为了测定样本中的精子浓度，用500μl戊二醛稀释0.5μl精液。将10μl稀释液等分用于细胞计数。平均计数为4.97x10（9）+/-3.46精子/ml。使用曙红-黑松香染料进行形态学分析；20.33%的精子死亡。其他79.67%的活精子平均长度约为30μm，头部直径为4.488+/-0.7μm；鞭毛加中段长度为26.071+/-12.4μm。在这些精子中，69%的精子形态正常，31%的精子有原发性和继发性异常。观察到的异常率对该物种的人工受精潜力没有不利影响。描述一个物种的精液特征是人工繁殖圈养鱼类所需的最重要的信息集之一。它还对研究物种的总体生物学知识做出了重大贡献。

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