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首页> 外文期刊>Zygote >Development of prepubertal goat oocytes after their in vitro maturation and chemical activation
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Development of prepubertal goat oocytes after their in vitro maturation and chemical activation

机译：在体外成熟和化学活化后开发预接种山羊卵母细胞

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Experiments were conducted to study in vitro maturation of prepubertal goat oocytes and their developmental potential after chemical activation. In Experiment 1, cumulus-oocytes complexes collected from the ovaries of prepubertal goats slaughtered at a local abattoir were matured in vitro in TCM-199-based medium supplemented with 10 mu g/ml luteinizing hormone (LH) (treatment 1) or 10 mu g/ml LH + 0.1 mM l-cysteine (treatment 2). In Experiment 2, mature oocytes were activated with either 5 mu M ionomycin or 7% ethanol. After 18 h, some oocytes were randomly fixed and stained to evaluate their chromatin status, while others were cultured in embryo culture medium to study their further development. In Experiment 3, oocytes activated with 5 mu M ionomycin were cultured for 7 days in one of the four different culture media [Charles Rosenkrans medium (CR-1), TCM-199, potassium simplex optimization medium (KSOM) and synthetic oviductal fluid (SOF)] to study their developmental potential. The maturation rate in control, treatment 1, and treatment 2 media did not differ from each other (P > 0.05). However, the lowest degeneration of oocytes was observed in treatment 3 (P < 0.05) when compared with the other two groups. The proportion of activated oocytes was higher, while non-activated oocytes were lower in ionomycin group when compared with the group activated with ethanol (P < 0.05). The proportions of oocytes cleaved were 65.7, 56.8, 61.0 and 54.4% in CR-1, TCM-199, KSOM and SOF medium, respectively, with no significant difference. However, further development of cleaved oocytes was better in KSOM followed by SOF.
机译：本实验旨在研究山羊青春期前卵母细胞的体外成熟及其化学活化后的发育潜能。在实验1中，从在当地屠宰场屠宰的青春期前山羊卵巢中收集的卵丘卵母细胞复合物在添加10μg/ml促黄体生成素（LH）（处理1）或10μg/ml LH+0.1 mM l-半胱氨酸（处理2）的TCM-199基培养基中体外成熟。在实验2中，成熟卵母细胞被5μM离子霉素或7%乙醇激活。18小时后，一些卵母细胞被随机固定并染色以评估其染色质状态，而另一些卵母细胞在胚胎培养基中培养以研究其进一步发育。在实验3中，用5μM离子霉素激活的卵母细胞在四种不同的培养基（Charles Rosenkrans培养基（CR-1）、TCM-199、单一钾优化培养基（KSOM）和合成输卵管液（SOF））中培养7天，以研究其发育潜力。对照组、处理1和处理2培养基中的成熟率没有差异（P>0.05）。然而，与其他两组相比，处理3的卵母细胞退化程度最低（P<0.05）。与乙醇激活组相比，离子霉素组激活卵母细胞比例较高，而非激活卵母细胞比例较低（P<0.05）。在CR-1、TCM-199、KSOM和SOF培养基中，卵裂率分别为65.7%、56.8%、61.0%和54.4%，差异无显著性。然而，在KSOM中，卵裂卵母细胞的进一步发育更好，其次是SOF。

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