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首页> 外文期刊>Domestic Animal Endocrinology >Understanding the influence of trenbolone acetate and polyamines on proliferation of bovine satellite cells
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Understanding the influence of trenbolone acetate and polyamines on proliferation of bovine satellite cells

机译：了解醋酸醋酸甲酮和多胺对牛卫星细胞增殖的影响

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Approximately 90% of beef cattle on feed in the United States receive at least one anabolic implant, which results in increased growth, efficiency, and economic return to producers. However, the complete molecular mechanism through which anabolic implants function to improve skeletal muscle growth remains unknown. This study had 2 objectives: (1) determine the effect of polyamines and their precursors on proliferation rate in bovine satellite cells (BSC); and (2) understand whether trenbolone acetate (TBA), a testosterone analog, has an impact on the polyamine biosynthetic pathway. To address these, BSC were isolated from 3 finished steers and cultured. Once cultures reached 75% confluency, they were treated in 1% fetal bovine serum (FBS) and/or 10 nM TBA, 10 mM methionine (Met), 8 mM ornithine (Orn), 2 mM putrescine (Put), 1.5 mM spermidine (Spd), or 0.5 mM spermine (Spe). Initially, a range of physiologically relevant concentrations of Met, Orn, Put, Spd, and Spe were tested to determine experimental doses to implement the aforementioned experiments. One, 12, or 24 h after treatment, mRNA was isolated from cultures and abundance of paired box transcription factor 7 (Pax7), Sprouty 1 (Spry), mitogenactivated protein kinase-1 (Mapk), ornithine decarboxylase (Odc), and S adenosylmethionine (Amd1) were determined, and normalized to 18S. No treatment x time interactions were observed (P >= 0.05). Treatment with TBA, Met, Orn, Put, Spd, or Spe increased (P <= 0.05) BSC proliferation when compared with control cultures. Treatment of cultures with Orn or Met increased (P <= 0.01) expression of Odc 1 h after treatment when compared with control cultures. Abundance of Amd1 was increased (P < 0.01) 1 h after treatment cultures treated with Spd or Spe when compared with 1% FBS controls. Cultures treated with TBA had increased (P < 0.01) abundance of Spry mRNA 12 h after treatment, as well as increased mRNA abundance of Mapk (P < 0.01) 12 h and 24 h after treatment when compared with 1% FBS control cultures. Treatment with Met increased (P < 0.01) mRNA abundance of Pax7 1 h after treatment as compared with 1% FBS controls. These results indicate that treatments of BSC cultures with polyamines and their precursors increase BSC proliferation rate, as well as abundance of mRNA involved in cell proliferation. In addition, treatment of BSC cultures with TBA, polyamines, or polyamine precursors impacts expression of genes related to the polyamine biosynthetic pathway and proliferation. (C) 2020 The Authors. Published by Elsevier Inc.
机译：在美国，大约90%的食用肉牛至少接受了一种合成代谢植入物，从而提高了生长、效率和生产者的经济回报。然而，合成代谢植入物改善骨骼肌生长的完整分子机制尚不清楚。本研究有2个目的：（1）研究多胺及其前体对牛卫星细胞（BSC）增殖率的影响；（2）了解睾酮类似物醋酸川波酮（TBA）是否对多胺生物合成途径有影响。为了解决这些问题，从3头成品阉牛中分离并培养BSC。一旦培养物达到75%的融合率，它们被处理在1%胎牛血清（FBS）和/或10 nM TBA、10 mM蛋氨酸（Met）、8 mM鸟氨酸（Orn）、2 mM腐胺（Put）、1.5 mM亚精胺（Spd）或0.5 mM亚精胺（Spe）中。最初，测试了一系列与生理相关的Met、Orn、Put、Spd和Spe浓度，以确定实施上述实验的实验剂量。治疗后1小时、12小时或24小时，从培养物中分离mRNA，测定配对盒转录因子7（Pax7）、Sprouty 1（Spry）、有丝分裂激活蛋白激酶-1（Mapk）、鸟氨酸脱羧酶（Odc）和S腺苷蛋氨酸（Amd1）的丰度，并将其标准化为18S。未观察到治疗与时间的相互作用（P>=0.05）。与对照培养相比，TBA、Met、Orn、Put、Spd或Spe处理增加了BSC的增殖（P<=0.05）。与对照培养物相比，用Orn或Met处理培养物后1小时Odc的表达增加（P<0.01）。与1%的FBS对照组相比，经Spd或Spe处理的培养物处理后1h，Amd1的丰度增加（P<0.01）。与1%胎牛血清对照培养物相比，经TBA处理的培养物在处理12小时后Spry mRNA丰度增加（P<0.01），以及在处理12小时和24小时后Mapk mRNA丰度增加（P<0.01）。与1%FBS对照组相比，Met治疗后1小时Pax7 mRNA丰度增加（P<0.01）。这些结果表明，用多胺及其前体处理BSC培养物可提高BSC的增殖率，并增加参与细胞增殖的大量mRNA。此外，用TBA、多胺或多胺前体处理BSC培养物会影响多胺生物合成途径和增殖相关基因的表达。（C） 2020年，作者。爱思唯尔公司出版。

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