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首页> 外文期刊>Journal of biochemical and molecular toxicology >An in vivo and in vitro comparison of the effects of amoxicillin, gentamicin, and cefazolin sodium antibiotics on the mouse hepatic and renal glutathione reductase enzyme
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An in vivo and in vitro comparison of the effects of amoxicillin, gentamicin, and cefazolin sodium antibiotics on the mouse hepatic and renal glutathione reductase enzyme

机译：体内和体外比较阿莫西林，庆大霉素和头孢唑啉钠抗生素对小鼠肝癌和肾谷胱甘肽还原酶酶的影响

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Despite the fact that the use of antibiotics is increasing worldwide, it is clear that antibiotics can lead to oxidative stress. This is the first study to make a comparison of the impact of frequently prescribed antibiotics, including amoxicillin, gentamicin, and cefazolin sodium, on the gene, protein, and activity of glutathione reductase (GR), which is one of the primary antioxidant enzymes, in mouse liver and kidney tissues. First, the GR enzyme was purified by the 2',5'-ADP Sepharose 4B affinity chromatography with a specific activity of 84.615 EU/mg protein and 9.63 EU/mg protein from the mouse liver and kidney, respectively. The in vitro inhibitory effects of the antibiotics in question was determined. While cefazolin sodium did not exhibit any inhibitory effect, gentamicin and amoxicillin inhibited GR activity in both tissues. Furthermore, the in vivo effects of these drugs were investigated, and amoxicillin and cefazolin sodium-inhibited GR activity in both liver and kidney tissues, while gentamicin did not have any effect on the kidney. Besides, while gentamicin downregulated and cefazolin sodium upregulated Gr gene expression, amoxicillin did not alter it. Protein expression was only affected by the administration of cefazolin sodium in the kidney. This study is important as it demonstrates that while amoxicillin and gentamicin showed parallel effects on the GR activity in liver and kidney tissues both in vitro and in vivo, cefazolin sodium had a very strong effect on hepatic and renal GR in vivo. Furthermore, the antibiotics used in this study induced oxidative stress in both tissues.
机译：尽管抗生素的使用在世界范围内不断增加，但很明显，抗生素会导致氧化应激。这是首次比较常用抗生素（包括阿莫西林、庆大霉素和头孢唑林钠）对小鼠肝和肾组织中主要抗氧化酶之一谷胱甘肽还原酶（GR）的基因、蛋白质和活性的影响的研究。首先，通过2'，5'-ADP-Sepharose 4B亲和层析纯化小鼠肝脏和肾脏中的GR酶，其比活性分别为84.615 EU/mg蛋白质和9.63 EU/mg蛋白质。确定了有关抗生素的体外抑制作用。头孢唑林钠无任何抑制作用，庆大霉素和阿莫西林抑制两种组织的GR活性。此外，还研究了这些药物的体内效应，阿莫西林和头孢唑林钠抑制肝脏和肾脏组织中的GR活性，而庆大霉素对肾脏没有任何影响。此外，庆大霉素下调Gr基因表达，头孢唑林钠上调Gr基因表达，而阿莫西林没有改变Gr基因表达。蛋白质表达仅受肾内注射头孢唑林钠的影响。这项研究很重要，因为它表明，虽然阿莫西林和庆大霉素在体外和体内对肝和肾组织中的GR活性表现出平行效应，但头孢唑林钠在体内对肝和肾GR具有非常强的作用。此外，本研究中使用的抗生素在两种组织中都会引起氧化应激。

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