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首页> 外文期刊>Journal of biochemical and molecular toxicology >Ameliorating effects of agomelatine on testicular and epididymal damage induced by methotrexate in rats
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Ameliorating effects of agomelatine on testicular and epididymal damage induced by methotrexate in rats

机译：胍葡萄酒对大鼠甲氨蝶呤诱导睾丸和附睾损伤的改善作用

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摘要

The aim of this study was to investigate the possible prophylactic effects of agomelatine (AGO) against testicular and epididymal damage induced by methotrexate (MTX) in rats. Twenty-four male Wistar albino rats were divided into three groups: Group I (control group), Group II (MTX group: 20 mg/kg MTX, i.p, single dose), and Group III (MTX+AGO group: 20mg/kg MTX, i.p, single dose+40mg/kg AGO; gavage, 7 days). The rats were killed under anesthesia 24 hours after the last AGO application. Testicular and epididymal tissues were bilaterally removed for morpho-metric, biochemical, pathological, and immunohistochemical analyses. Body, testicular, and epididymal weights were measured. Malondialdehyde (MDA), superoxide dismutase, catalase, and glutathione peroxidase levels were measured in testes. Sperm count, hyperemia, edema, inflammatory reaction, degenerated and necrotic cells were evaluated by histopathological analysis. In addition, inducible nitric oxide synthase (iNOS), granulocyte colony-stimulating factor (G-CSF), osteopontin (OPN), and heat shock protein-70 (HSP70) immune reactions were analyzed in testes and epididymides. Decreased epididymal weights, increased MDA levels, decreased sperm count, hyperemia, edema, inflammatory reaction, and degenerated and necrotic cells were observed in the MTX group. In addition, iNOS, HSP70, G-CSF, and OPN immune reactions were increased. AGO improved morphometric, biochemical, histopathological, and immunohistochemical findings. The present study confirms that MTX induces testicular and epididymal damage both biochemically and immunohisto-chemically. However, AGO demonstrated ameliorative effects on both biochemical and pathological findings of the current study.
机译：本研究的目的是探讨阿戈美拉汀（AGO）对甲氨蝶呤（MTX）诱导的大鼠睾丸和附睾损伤的可能预防作用。24只雄性Wistar白化大鼠分为三组：第一组（对照组）、第二组（MTX组：20mg/kg MTX，I.p，单次给药）和第三组（MTX+AGO组：20mg/kg MTX，I.p，单次给药+40mg/kg AGO；灌胃，7天）。最后一次用药24小时后，在麻醉下处死大鼠。双侧切除睾丸和附睾组织，进行形态计量学、生化、病理学和免疫组化分析。测量身体、睾丸和附睾重量。检测睾丸中丙二醛（MDA）、超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的水平。通过组织病理学分析评估精子计数、充血、水肿、炎症反应、变性和坏死细胞。此外，还分析了睾丸和附睾中的诱导型一氧化氮合酶（iNOS）、粒细胞集落刺激因子（G-CSF）、骨桥蛋白（OPN）和热休克蛋白-70（HSP70）免疫反应。MTX组附睾重量减少，MDA水平升高，精子计数减少，充血、水肿、炎症反应，细胞变性和坏死。此外，iNOS、HSP70、G-CSF和OPN免疫反应增加。AGO改善了形态计量学、生物化学、组织病理学和免疫组化结果。目前的研究证实，MTX在生物化学和免疫组织化学方面都会导致睾丸和附睾损伤。然而，AGO对当前研究的生化和病理结果都有改善作用。

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