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首页> 外文期刊>Journal of biochemical and molecular toxicology >Zingerone suppresses cell proliferation via inducing cellular apoptosis and inhibition of the PI3K/AKT/mTOR signaling pathway in human prostate cancer PC-3 cells
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Zingerone suppresses cell proliferation via inducing cellular apoptosis and inhibition of the PI3K/AKT/mTOR signaling pathway in human prostate cancer PC-3 cells

机译：条形酮通过诱导细胞凋亡和抑制人前列腺癌PC-3细胞中PI3K / AKT / MTOR信号传导途径的诱导细胞凋亡和抑制细胞增殖

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摘要

Prostate cancer (PCa) is both the foremost and second cause of cancer death in the male population. Patients with hormone-dependent PCa are initially sensitive to androgen-deprivation therapy, later the cancer progress to a hormone-independent state and fails to respond and progress to the metastatic stage, where the cells gain the ability to escape cell death and develop resistance to current therapies, thereby leading to migration, invasion, and metastasis of cancer. Many clinical trials using nutraceuticals on cancer using human subjects have also been extensively studied, these studies confirm the efficacy of drugs tested in in vitro and in vivo preclinical models. Among various dietary phytochemicals, ginger is commonly used in the diet and possesses many active principles that act against cancer. Among various active principles, zingerone is a key active phenolic compound present in Zingiber officinale (Ginger), it has potent antioxidant property and it acts against carcinogens. The present study evaluated the efficacy of zingerone at different doses on the PCa cell line regarding apoptosis, upstream signing molecules such as Akt/mTOR, and migration metastasis. A cell viability assay using MTT was performed to estimate the percentage of viability of zingerone-treated PC-3 cells. The mitochondrial membrane potential, intracellular reactive oxygen species, and apoptosis induction in the zingerone-treated PC-3 cells were studied by using different fluorescence staining techniques. The expression patterns of PI3K, AKT, p-AKT, mTOR, and p-mTOR were investigated through the Western blot analysis assay. Zingerone induces apoptosis and alters Akt/mTOR molecules; it also inhibits cell adhesion and migration of PCa cells. From the present study, it is concluded that zingerone effectively induces apoptosis and inhibits cancer signaling, thereby acting as a potent drug against PCa.
机译：前列腺癌（PCa）是男性癌症死亡的首要和第二大原因。激素依赖性前列腺癌患者最初对雄激素剥夺疗法敏感，后来癌症发展到激素非依赖状态，没有反应并进展到转移阶段，在转移阶段，细胞获得逃避细胞死亡的能力，并对当前疗法产生耐药性，从而导致癌症的迁移、侵袭和转移。许多使用营养药物治疗癌症的临床试验也已经被广泛研究，这些研究证实了药物在体外和体内临床前模型中的有效性。在各种饮食植物化学物质中，生姜被广泛用于饮食中，并具有许多抗癌的活性成分。在各种活性成分中，生姜酮是生姜中的一种关键活性酚类化合物，具有很强的抗氧化性和抗致癌作用。本研究评估了不同剂量的锌酮对PCa细胞系在凋亡、上游信号分子（如Akt/mTOR）和转移方面的疗效。采用MTT法进行细胞活力测定，以估计经锌酮处理的PC-3细胞的活力百分比。采用不同的荧光染色技术研究了锌酮处理的PC-3细胞的线粒体膜电位、细胞内活性氧和凋亡诱导。通过westernblot分析研究PI3K、AKT、p-AKT、mTOR和p-mTOR的表达模式。锌酮诱导细胞凋亡并改变Akt/mTOR分子；它还抑制PCa细胞的粘附和迁移。从目前的研究中可以得出结论，锌酮能有效诱导细胞凋亡并抑制癌症信号传导，从而作为一种有效的抗前列腺癌药物。

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