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首页> 外文期刊>Journal of biochemical and molecular toxicology >Trif lumuron induces cytotoxic effects on hepatic and renal human cell lines
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Trif lumuron induces cytotoxic effects on hepatic and renal human cell lines

机译：Trif Lumuron诱导对肝癌和肾脏人细胞系的细胞毒性作用

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摘要

Insect growth regulator insecticides are a new class of pesticides, commonly used around the world to control insect damages. Among those compounds, we focused our interest on triflumuron (TFM), which is less toxic than other conventional insecticides. However, not much is known about its toxic effects on mammalian systems. Therefore, our study aimed toward evaluating the cytotoxic and genotoxic effects of TFM using two different cell lines, the human renal embryonic cells (HEK 293) and hepatocytes (Hep G2). We showed, according to the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, that TFM reduced significantly the cell viability and increased the reactive oxygen species generation, malondialdehyde levels, and mitochondrial membrane potential in both cell lines. The antioxidant system was disturbed as assessed by the increased activities in both catalase and superoxide dismutase. We demonstrated also, that TFM is an inductor of DNA damages quantified by the comet assay. Moreover, we showed an overexpression of proapoptotic Bax and a decrease in antiapoptotic Bcl-2 expression. As a conclusion, we demonstrate that the liver presents the major target organ to TFM, in which the cytotoxicity and the genotoxic effects were significantly higher in hepatic cells than in renal cells and by consequence its uses must be controlled.
机译：昆虫生长调节剂杀虫剂是一类新型杀虫剂，在世界各地广泛用于控制昆虫危害。在这些化合物中，我们主要关注三氟脲（TFM），它比其他常规杀虫剂毒性更小。然而，人们对其对哺乳动物系统的毒性影响知之甚少。因此，我们的研究旨在使用两种不同的细胞系，即人类肾胚胎细胞（HEK 293）和肝细胞（Hep G2），评估TFM的细胞毒性和遗传毒性作用。根据3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴化分析，我们发现TFM显著降低了两种细胞系的细胞活力，增加了活性氧生成、丙二醛水平和线粒体膜电位。过氧化氢酶和超氧化物歧化酶活性的增加对抗氧化系统造成了干扰。我们还证明，TFM是彗星试验量化的DNA损伤的诱导因子。此外，我们还发现促凋亡Bax的过度表达和抗凋亡Bcl-2表达的降低。作为结论，我们证明肝脏是TFM的主要靶器官，其中肝细胞的细胞毒性和遗传毒性效应显著高于肾细胞，因此必须控制其用途。

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