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首页> 外文期刊>Journal of biochemical and molecular toxicology >Quercetin alleviated H_2O_2-inclucecl apoptosis and steroidogenic impairment in goat luteinized granulosa cells
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Quercetin alleviated H_2O_2-inclucecl apoptosis and steroidogenic impairment in goat luteinized granulosa cells

机译：槲皮素缓解了H_2O_2-Chaptucecl凋亡和山羊叶氏鳞状细胞细胞中的甾体损伤

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摘要

Quercetin (Que) is a natural flavonoid in most plants. Luteinized granulosa cell (LGC) culture is necessary for the study of follicle growth/differentiation. In the present study, we analyzed the role of Que in steroid production and apoptosis in hydrogen peroxide (H2O2)-treated goat LGCs. The results showed that treatment with H2O2 induced apoptosis in goat LGCs, and treatment with Que decreased LGC apoptosis induced by H2O2 (P < .05), accompanied with the different expressions of BAX, BCL-2, Caspase 3, and Cleaved caspase 3. Meanwhile, the messenger RNA expressions of nuclear factor erythroid 2 like 2 [Nrf2) and its downstream genes were upregulated with H2O2 +Que treatment, accompanied by the increased cellular viability (P < .05). Furthermore, Que alleviated H2O2-induced reduction in the secretion of progesterone (P4) (P < .05); however, it had no effect on the secretion of estrogen (E2). Simultaneously, the expressions of StAR and P450scc were increased when treated with Que +H2O2, compared with the group treated with only H2O2 (P< .05). In conclusion, it is observed that Que could alleviate the H2O2-induced apoptosis and steroidogenic impairment in goat LGCs, which might be mediated by the Nrf2 pathway.
机译：槲皮素（Quercetin，Que）是大多数植物中的一种天然类黄酮。黄体化颗粒细胞（LGC）培养对于卵泡生长/分化的研究是必要的。在本研究中，我们分析了Que在过氧化氢（H2O2）处理的山羊LGC类固醇生成和凋亡中的作用。结果表明，H2O2诱导山羊LGC凋亡，Que降低H2O2诱导的LGC凋亡（P<0.05），并伴有BAX、BCL-2、Caspase 3和裂解的Caspase 3的不同表达。同时H2O2+Que处理可上调核因子红系2样2[Nrf2]及其下游基因的信使RNA表达，同时提高细胞活力（P<0.05）。此外，Que可减轻H2O2诱导的孕酮（P4）分泌减少（P<0.05）；但对雌激素（E2）分泌无影响同时，与仅用H2O2处理组相比，Que+H2O2处理组的StAR和P450scc表达增加（P<0.05）。总之，观察到Que可以减轻H2O2诱导的山羊LGC凋亡和类固醇生成障碍，这可能是通过Nrf2途径介导的。

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