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首页> 外文期刊>Journal of biochemical and molecular toxicology >Docosahexaenoic acid inhibits Ca~2+ influx and downregulates CaSR by upregulating microRNA-16 in pulmonary artery smooth muscle cells
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Docosahexaenoic acid inhibits Ca~2+ influx and downregulates CaSR by upregulating microRNA-16 in pulmonary artery smooth muscle cells

机译：DocosaheNoice酸抑制Ca〜2 +流入，通过上调MicroRNA-16在肺动脉平滑肌细胞中下调Casr

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摘要

Docosahexaenoic acid (DHA) is reported to have the potential to ameliorate pulmonary arterial hypertension (PAH), while the specific mechanism is still obscure. This study aims to investigate the function of DHA in pulmonary artery smooth muscle cells (PASMCs) and explore the underlying mechanism. In our study, DHA was used to incubate PASMCs. Cytosolic-free Ca~2+ concentration ([Ca~2+]cyt) was measured using Fluo-3 AM method. Real-time polymerase chain reaction was used to detect microRNA-16 (miR-16) and calcium-sensing receptor (CaSR) messenger RNA expression levels. CCK-8 assay, BrdU assay, and Transwell assay were employed to detect the effects of DHA on proliferation and migration of PASMCs. CaSR was confirmed as a direct target of miR-16 using dual-luciferase assay, polymerase chain reaction, and Western blot analysis. It was found that DHA significantly inhibited PASMC proliferation and migration and decreased [Ca~2+]cyt. After trans-fection of miR-16 mimics, proliferation and migration ability of PASMCs were significantly inhibited, whereas opposite effects were observed after miR-16 inhibition. [Ca~2+]cyt was also inhibited by miR-16 transfection. DHA then promoted the expression of miR-16, and the effects of DHA on PASMCs were annulled when miR-16 was inhibited. CaSR was identified as a direct target of miR-16. CaSR was inhibited directly by miR-16 and indirectly by DHA. In conclusion, DHA inhibits the proliferation and migration of PASMCs, and probably ameliorates PAH via regulating miR-16/CaSR axis.
机译：据报道，二十二碳六烯酸（DHA）有可能改善肺动脉高压（PAH），但具体机制尚不清楚。本研究旨在研究DHA在肺动脉平滑肌细胞（PASMC）中的作用，并探讨其潜在机制。在我们的研究中，DHA被用于培养PASMC。用Fluo-3 AM法测定细胞内游离Ca~（2+）浓度（[Ca~（2+）]cyt）。实时聚合酶链反应检测microRNA-16（miR-16）和钙敏感受体（CaSR）信使RNA的表达水平。采用CCK-8法、BrdU法和Transwell法检测DHA对PASMC增殖和迁移的影响。通过双荧光素酶分析、聚合酶链反应和westernblot分析证实CaSR是miR-16的直接靶点。结果发现，DHA显著抑制PASMC的增殖和迁移，并降低[Ca~2+]cyt。在转染miR-16模拟物后，PASMC的增殖和迁移能力受到显著抑制，而在抑制miR-16后观察到相反的效果。mirca~2+也抑制了转染。DHA随后促进了miR-16的表达，当miR-16受到抑制时，DHA对PASMC的作用被取消。CaSR被确定为miR-16的直接靶点。miR-16直接抑制CaSR，DHA间接抑制CaSR。总之，DHA抑制PASMC的增殖和迁移，并可能通过调节miR-16/CaSR轴改善PAH。

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