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首页> 外文期刊>Journal of Biophotonics >In situ measurement of the isoplanatic patch for imaging through intact bone
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In situ measurement of the isoplanatic patch for imaging through intact bone

机译：以完整骨骼成像的Isoplanatic贴剂的原位测量

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Wavefront-shaping (WS) enables imaging through scattering tissues like bone, which is important for neuroscience and bone-regeneration research. WS corrects for the optical aberrations at a given depth and field-of-view (FOV) within the sample; the extent of the validity of which is limited to a region known as the isoplanatic patch (IP). Knowing this parameter helps to estimate the number of corrections needed for WS imaging over a given FOV. In this paper, we first present direct transmissive measurement of murine skull IP using digital optical phase conjugation based focusing. Second, we extend our previously reported phase accumulation ray tracing (PART) method to provide in-situin-silicoestimation of IP, called correlative PART (cPART). Our results show an IP range of 1 to 3 mu m for mice within an age range of 8 to 14 days old and 1.00 +/- 0.25 mu m in a 12-week old adult skull. Consistency between the two measurement approaches indicates that cPART can be used to approximate the IP before a WS experiment, which can be used to calculate the number of corrections required within a given field of view.
机译：波前整形（WS）可以通过骨等散射组织成像，这对神经科学和骨再生研究非常重要。WS校正样品内给定深度和视野（FOV）下的光学像差；其有效范围仅限于称为等平面面片（IP）的区域。了解该参数有助于估计在给定视野下WS成像所需的校正次数。在本文中，我们首次提出了使用基于数字光学相位共轭聚焦的小鼠头骨IP的直接透射测量。其次，我们扩展了之前报道的相位累积射线追踪（PART）方法，以提供IP的原位硅估计，称为相关部分（cPART）。我们的结果显示，8至14天大的小鼠的IP范围为1至3μm，12周大的成年头骨的IP范围为1.00+/-0.25μm。两种测量方法之间的一致性表明，在WS实验之前，cPART可用于近似IP，这可用于计算给定视野内所需的校正次数。

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