首页> 外文期刊>Journal of Crop Improvement >Genetic diversity among commercial arabica coffee {Coffea arabica L.) varieties in Ethiopia using simple sequence repeat markers
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Genetic diversity among commercial arabica coffee {Coffea arabica L.) varieties in Ethiopia using simple sequence repeat markers

机译:埃塞俄比亚商业阿拉伯咖啡{Coffea Arabica L.)品种的遗传多样性使用简单序列重复标记

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Ethiopia is the center of origin and genetic diversity of arabica coffee. Forty-two commercial arabica coffee varieties were developed by Jimma Agricultural Research Center (JARC) of Ethiopian Institute of Agricultural Research (EIAR) and released forproduction under diverse agro-ecologies of the country. Information on the level of genetic diversity among these varieties is scarce. Out of the 42 varieties, the genetic diversity of 40 widely cultivated commercial varieties was assessed using 14 simple sequence repeat (SSR) markers. These markers revealed polymorphism among the varieties. High average number of polymorphic alleles (7.5) and polymorphic information content (PIC = 80%) per locus were detected among the varieties. The genetic similarity among varieties using the Jaccard's similarity coefficient ranged from 0.14 to 0.78, with a mean of 0.38. The range of genetic similarity coefficient values in 92% of the possible pair-wise combinations varied from 0.14 to 0.50, indicating the presenceof distant genetic relatedness among the varieties. Unweighted pair group method using arithmetic mean (UPGMA) clustering showed six major clusters and three singletons. Coffee varieties, belonging to the same geographic origin, were distributed acrossclusters. This study represents the first evidence of the presence of a high level of genetic diversity in Ethiopian commercial arabica coffee varieties. Divergent varieties with complementing traits could be crossed to develop productive hybrid coffee varieties.
机译:埃塞俄比亚是阿拉比卡咖啡的起源和遗传多样性中心。42个商业阿拉比卡咖啡品种由埃塞俄比亚农业研究所(EIAR)的吉玛农业研究中心(JARC)开发,并在该国不同的农业生态环境下进行生产。关于这些品种的遗传多样性水平的信息很少。在42个品种中,使用14个简单序列重复(SSR)标记评估了40个广泛栽培的商业品种的遗传多样性。这些标记揭示了品种间的多态性。在这些品种中,每个位点的平均多态性等位基因数(7.5)和多态性信息含量(PIC=80%)较高。利用Jaccard相似系数,品种间的遗传相似性在0.14到0.78之间,平均值为0.38。92%的可能成对组合的遗传相似系数范围在0.14到0.50之间,表明品种间存在远缘遗传相关。使用算术平均(UPGMA)聚类的未加权对群方法显示六个主要聚类和三个单态。咖啡品种属于同一地理起源,分布在不同的聚类中。这项研究首次证明埃塞俄比亚商品阿拉比卡咖啡品种具有高度的遗传多样性。具有互补性状的不同品种可以杂交,以培育高产杂交咖啡品种。

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