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首页> 外文期刊>Journal of Experimental Botany >The chaperonin 60 protein SlCpn60a1 modulates photosynthesis and photorespiration in tomato
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The chaperonin 60 protein SlCpn60a1 modulates photosynthesis and photorespiration in tomato

机译：伴侣素60蛋白SLCPN60A1在番茄中调节光合作用和光素

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Photosynthesis, an indispensable biological process of plants, produces organic substances for plant growth, during which photorespiration occurs to oxidize carbohydrates to achieve homeostasis. Although the molecular mechanism underlying photosynthesis and photorespiration has been widely explored, the crosstalk between the two processes remains largely unknown. In this study, we isolated and characterized a T-DNA insertion mutant of tomato (Solanum lycopersicum) named yellow leaf (yl) with yellowish leaves, retarded growth, and chloroplast collapse that hampered both photosynthesis and photorespiration. Genetic and expression analyses demonstrated that the phenotype of yl was caused by a loss-of-function mutation resulting from a single-copy T-DNA insertion in chaperonin 60a1 (SlCPN60a1). SlCPN60a1 showed high expression levels in leaves and was located in both chloroplasts and mitochondria. Silencing of SlCPN60a1using virus-induced gene silencing and RNA interference mimicked the phenotype of yl. Results of two-dimensional electrophoresis and yeast two-hybrid assays suggest that SlCPN60a1 potentially interacts with proteins that are involved in chlorophyll synthesis, photosynthetic electron transport, and the Calvin cycle, and further affect photosynthesis. Moreover, SlCPN60a1 directly interacted with serine hydroxymethyltransferase (SlSHMT1) in mitochondria, thereby regulating photorespiration in tomato. This study outlines the importance of SlCPN60a1 for both photosynthesis and photorespiration, and provides molecular insights towards plant genetic improvement.
机译：光合作用是植物不可或缺的生物过程，为植物生长产生有机物质，在此过程中，光呼吸发生，氧化碳水化合物以实现体内平衡。尽管光合作用和光呼吸的分子机制已被广泛探索，但这两个过程之间的相互作用仍基本未知。在这项研究中，我们分离并鉴定了番茄（Solanum lycopersicum）的T-DNA插入突变体黄叶（yl），黄叶淡黄色，生长迟缓，叶绿体塌陷，阻碍光合作用和光呼吸。遗传和表达分析表明，yl的表型是由伴侣蛋白60a1（SlCPN60a1）中单拷贝T-DNA插入导致的功能缺失突变引起的。SlCPN60a1在叶片中表现出高表达水平，位于叶绿体和线粒体中。使用病毒诱导的基因沉默和RNA干扰沉默SLCPN60A1可以模拟yl的表型。二维电泳和酵母双杂交分析的结果表明，SlCPN60a1可能与参与叶绿素合成、光合电子传递和卡尔文循环的蛋白质相互作用，并进一步影响光合作用。此外，SlCPN60a1直接与线粒体中的丝氨酸羟甲基转移酶（SlSHMT1）相互作用，从而调节番茄的光呼吸。本研究概述了SlCPN60a1对光合作用和光呼吸的重要性，并为植物遗传改良提供了分子见解。

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