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首页> 外文期刊>Journal of genetics >Identification of genomic regions associated with early plant vigour in lentil (Lens culinaris)
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Identification of genomic regions associated with early plant vigour in lentil (Lens culinaris)

机译：扁豆植物早期植物活力的基因组区域鉴定（镜片玉米糖）

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Lentil is one of the most important food legume species, however its genetic and genomic resources remained largely uncharacterized and unexploited. In the past few years, a number of genetic maps have been constructed and marker resources have been developed in lentil. These resources could be exploited for understanding the extent and distribution of genetic variation in genus Lens and also for developing saturated and consensus genetic maps suitable for quantitative trait loci (QTL) mapping and marker-assisted selection. The present study aims to enrich polymerase chain reaction-based linkage map of F-10 recombinant inbred lines (RILs) population of 94 individuals derived from cross WA8649090 x Precoz and identification of QTLs linked to early plant vigour traits. Of the 268 polymorphic markers (93 simple sequence repeats (SSR), three inter-simple sequence repeats (ISSRs) and 172 random amplified polymorphic DNA (RAPDs)), 265 (90 SSRs, three ISSRs and 172 RAPDs) were mapped on seven linkage groups, varying in length between 25.6 and 210.3 cM, coverage of 809.4 cM with an average marker spacing of 3.05 cM. The study also reported assigning of 24 new cross-genera SSRs of Trifolium pratense on the present linkage map. The RILs along with the parents were screened for shoot length, root length, seedling length, dry weight, number of leaves and number of branches based on two replications under polyhouse conditions. A QTL-hotspot consisting of six QTLs for shoot length (cm), root length (cm) and seedling length (cm) was observed between a map distances of 56.61 and 86.81 cM on LG1.
机译：小扁豆是最重要的食用豆科植物之一，但其遗传和基因组资源基本上尚未被描述和利用。在过去的几年里，人们在小扁豆中构建了许多遗传图谱，并开发了标记资源。这些资源可用于了解透镜属遗传变异的程度和分布，以及开发适合数量性状位点（QTL）定位和标记辅助选择的饱和和一致遗传图谱。本研究旨在丰富基于聚合酶链反应的F-10重组自交系（RIL）群体连锁图谱，该群体由来自WA8649090 x Precoz杂交的94个个体组成，并鉴定与早期植株活力性状相关的QTL。在268个多态性标记（93个简单序列重复（SSR）、3个简单序列间重复（ISSR）和172个随机扩增多态性DNA（RAPD））中，265个（90个SSR、3个ISSR和172个RAPD）被定位在7个连锁群上，长度在25.6到210.3厘米之间，覆盖范围为809.4厘米，平均标记间距为3.05厘米。本研究还报告了在现有连锁图谱上分配的24个新的红车轴草杂交属SSR。在polyhouse条件下，基于两次重复，对RIL和亲本进行了芽长、根长、幼苗长、干重、叶片数和分枝数的筛选。在LG1上，在56.61和86.81 cm的图谱距离之间观察到一个QTL热点，由6个涉及地上部长度（cm）、根系长度（cm）和幼苗长度（cm）的QTL组成。

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