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首页> 外文期刊>Journal of genetics >Molecular cloning, expression and mimicking antiviral activity analysis of retinoic acid-inducible gene-I in duck (Anas platyrhynchos)
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Molecular cloning, expression and mimicking antiviral activity analysis of retinoic acid-inducible gene-I in duck (Anas platyrhynchos)

机译：鸭子视黄酸诱导基因-I的分子克隆，表达和模拟抗病毒活性分析（Anas platynchos）

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摘要

Intracellular double-stranded RNA (dsRNA) is a chief sign of replication for many viruses. Pattern recognition receptors (PRRs) of the innate immune system detected the dsRNA and initiate the antiviral responses. Retinoic acid-inducible gene I (RIG-I), a member of PRRs, plays an essential regulatory role in dsRNA-induced signalling. In this study, the full-length complementary DNA (cDNA) of duck RIG-I (duRIG-I) was cloned using the reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of the cDNA ends (RACE). The cDNA of duRIG-I contained 97-bp 5 ' UTR, 141-bp 3 '-UTR and 2802 bp complete open-reading frame (ORF) encoding 933 amino acids. Multiple sequence alignments showed that duRIG-I shared high similarity with RIG-I from other vertebrates. Quantitative real-time PCR (qRT-PCR) analysis revealed that duRIG-I mRNA was expressed in all tested tissues, with high levels in the liver, heart, spleen, kidney and thymus, while lower in the duodenum. duRIG-I could be induced by treatment with poly(I:C). Further, overexpression of duRIG-I significantly activated the transcription of poly(I:C)-induced IFN-beta, IRF7, TRIF, Mx, STAT1 and STAT2 mRNA, and duRIG-I knockdown showed the opposite results. Overall, our results suggested that duRIG-I could be an important receptor for mimicking antiviral state in duck, which warrant further studies to show the possible mechanism.
机译：细胞内双链RNA（dsRNA）是许多病毒复制的主要标志。固有免疫系统的模式识别受体（PRR）检测到dsRNA并启动抗病毒反应。视黄酸诱导基因I（RIG-I）是PRRs的成员，在dsRNA诱导的信号传导中起着重要的调节作用。本研究采用逆转录聚合酶链反应（RT-PCR）和快速扩增cDNA末端（RACE）技术克隆了鸭RIG-I（duRIG-I）的全长互补DNA（cDNA）。duRIG-I的cDNA含有97bp的5′UTR、141bp的3′UTR和2802bp的完整开放阅读框（ORF），编码933个氨基酸。多重序列比对显示，duRIG-I与其他脊椎动物的RIG-I具有高度相似性。定量实时PCR（qRT PCR）分析显示，duRIG-I mRNA在所有受试组织中均有表达，在肝脏、心脏、脾脏、肾脏和胸腺中表达较高，而在十二指肠中表达较低。聚（I:C）处理可诱导duRIG-I。此外，duRIG-I的过度表达显著激活了多聚（I:C）诱导的IFN-β、IRF7、TRIF、Mx、STAT1和STAT2 mRNA的转录，duRIG-I的敲除显示了相反的结果。总的来说，我们的结果表明duRIG-I可能是模拟鸭体内抗病毒状态的一个重要受体，这需要进一步的研究来证明其可能的机制。

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  • 来源
    《Journal of genetics》 |2020年第1期|共9页
  • 作者

    Gu Tiantian; Li Guoqin; Tian Yong; Chen Li; Wu Xinsheng; Zeng Tao; Xu Qi; Vladyslav Spyrydonov; Chen Guohong; Lu Lizhi;

  • 作者单位

    Yangzhou Univ Jiangsu Key Lab Anim Genet Breeding &

    Mol Design Yangzhou 225009 Jiangsu Peoples R China;

    Zhejiang Acad Agr Sci Inst Anim Husb &

    Vet Med Hangzhou 310021 Zhejiang Peoples R China;

    Zhejiang Acad Agr Sci Inst Anim Husb &

    Vet Med Hangzhou 310021 Zhejiang Peoples R China;

    Zhejiang Acad Agr Sci Inst Anim Husb &

    Vet Med Hangzhou 310021 Zhejiang Peoples R China;

    Yangzhou Univ Jiangsu Key Lab Anim Genet Breeding &

    Mol Design Yangzhou 225009 Jiangsu Peoples R China;

    Zhejiang Acad Agr Sci Inst Anim Husb &

    Vet Med Hangzhou 310021 Zhejiang Peoples R China;

    Yangzhou Univ Jiangsu Key Lab Anim Genet Breeding &

    Mol Design Yangzhou 225009 Jiangsu Peoples R China;

    Natl Univ Life &

    Environm Sci Ukraine UA-03041 Kiev Ukraine;

    Yangzhou Univ Jiangsu Key Lab Anim Genet Breeding &

    Mol Design Yangzhou 225009 Jiangsu Peoples R China;

    Zhejiang Acad Agr Sci Inst Anim Husb &

    Vet Med Hangzhou 310021 Zhejiang Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 遗传学;
  • 关键词

    duck; retinoic acid-inducible gene I; gene expression; antivirus; poly(I; C); Anas platyrhynchos;

    机译：鸭;视黄酸 - 诱导基因I;基因表达;抗病毒;聚（i;c）;anas platyrhynchos;

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