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Fiber analysis: Evaluation of screen printing fabric filters bags by three statistical approaches

机译:纤维分析:通过三种统计方法评估丝网印刷织物滤袋

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Measurements of fiber fractions using the Ankom filter bag system, and four types of filter bags were compared with results obtained employing the procedure indicated by the Association of Official Analytical Chemists (AOAC). To assess agreement between procedures, three statistical approaches were used. Twenty-three materials differing widely in cell wall content, and composition were evaluated. Filter bags evaluated were Ankom F-57 (porosity: 25 mu m), and three types of bags made in our laboratory with polyester screen printing fabric, porosities 45 mu m (B-120), 36 mu m (B-140), and 23 mu m (B-150). Amylase neutral detergent fiber organic matter basis (aNDFom), acid detergent fiber organic matter basis (ADFom), and sulfuric acid lignin (Lignin (sa)) values obtained using crucibles (C) were compared with data obtained using F-57, B-120, B-140 or B-150 bags, and fiber values obtained with F-57 were compared with data obtained employing B-120, B-140 or B-150 bags. Statistical approaches used were analysis of variance (ANOVA), regression analysis (Deming method), and the Bland-Altman method of differences. In the ANOVA, no differences (P>0.18) were observed, between measurements of aNDFom, ADFom, and Lignin (sa) performed employing the AOAC, or the Ankom procedure using any type of bag. In the regression analysis, in all associations tested, the 0.95 confidence interval of the slope of the regressions contained the value 1, and Pearson correlations (P<0.001) were greater than 0.94. In aNDFom, ADFom, and Lignin (sa) comparisons, five, three, and two of the seven regressions did not include 0 in the 0.95 confidence interval aide intercept. In the Bland-Altman method, proportion of differences between limits of agreement, ranged from 0.91 to 1.0, 0.91 to 0.96, and 0.74 to 0.96, respectively, in aNDFom. ADFom and Lignin (sa) method comparisons. The statistical approaches evaluated, suggested a reasonable degree of agreement between aNDFom, ADFom, and Lignin (so) measurements performed by the AOAC, and the Ankom Fiber Analyzer procedure, when F-57 or B-120 bags were used. This would suggest that using these procedures in sequential analysis, it is unlikely to yield erroneous values of the analytes. The Bland-Altman method resulted in a sensitive method, to identify differences among analytical procedures. Results suggest that, in the description of fiber protocols using the Ankom Fiber Analyzer, it is crucial to specifically describe filter bags employed
机译:使用Ankom滤袋系统和四种类型的滤袋对纤维组分的测量结果与采用官方分析化学家协会(AOAC)指示的程序进行了比较。为了评估程序之间的一致性,使用了三种统计方法。对二十三种材料的细胞壁含量和组成差异很大的材料进行了评估。评估的滤袋为Ankom F-57(孔隙度:25微米),以及在我们实验室中用聚酯丝网印刷织物制成的三种类型的滤袋,孔隙率分别为45微米(B-120),36微米(B-140),和23微米(B-150)。将使用坩埚(C)获得的淀粉酶中性洗涤剂纤维有机物(aNDFom),酸性洗涤剂纤维有机物(ADFom)和硫酸木质素(Lignin(sa))值与使用F-57,B-将120,B-140或B-150袋以及通过F-57获得的纤维值与使用B-120,B-140或B-150袋获得的数据进行比较。使用的统计方法是方差分析(ANOVA),回归分析(Deming方法)和Bland-Altman差异法。在ANOVA中,使用AOAC进行的aNDFom,ADFom和木质素(sa)的测量或使用任何类型袋子的Ankom程序之间均未观察到差异(P> 0.18)。在回归分析中,在所有测试的关联中,回归斜率的0.95置信区间包含值1,而Pearson相关性(P <0.001)大于0.94。在aNDFom,ADFom和Lignin(sa)的比较中,七个回归中的五个,三个和两个在0.95置信区间辅助截距中不包括0。在Bland-Altman方法中,在aNDFom中,一致极限之间差异的比例分别为0.91至1.0、0.91至0.96和0.74至0.96。 ADFom和木质素(sa)方法的比较。评估的统计方法表明,当使用F-57或B-120袋时,由AOAC执行的aNDFom,ADFom和木质素(so)测量与Ankom纤维分析仪程序之间的合理程度的一致性。这表明在顺序分析中使用这些程序,不太可能产生错误的分析物值。 Bland-Altman方法产生了一种灵敏的方法,可以识别分析程序之间的差异。结果表明,在使用Ankom光纤分析仪的光纤协议描述中,至关重要的是具体描述所使用的滤袋

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