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首页> 外文期刊>Journal of insect biotechnology and sericology >The physiological accumulation of mutant fibroin light chains induces an unfolded protein response in the posterior silk gland of the Sericin cocoon Nd-s~D strain of silkworm Bombyx mori
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The physiological accumulation of mutant fibroin light chains induces an unfolded protein response in the posterior silk gland of the Sericin cocoon Nd-s~D strain of silkworm Bombyx mori

机译：突变体纤维素光链的生理堆积在蚕豆菌菌菌菌菌菌菌的后丝腺体中诱导展开的蛋白质反应

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摘要

Fibroin, a major component of silk fiber, is composed of light (L) chains, heavy chains, and fhx/P25 in the silkworm Bombyx mori. Sericin cocoon (Nd-s°) is a silkworm strain expressing a mutant fibroin L chain that is accumulated in the endoplasmic reticulum (ER) of posterior silk glands (PSGs). However, little is known about the effects of accumulation in PSGs in Nd-s~D strain. We compared the PSG gene expression profiles of 5th-instar larvae of Nd-s~D strain and the fibroin-producing normal strain. cDNA representational difference analysis identified candidate genes whose expression levels were higher in Nd-s° strain than in normal strain. We focused on heat shock proteins and cathepsin B, a major lysosomal protease. We confirmed upregulation ofBiP(GRP78), Hsp20.8, and cathepsin B at the transcriptional and/or translational levels. These results suggest that the accumulation of mutant L chain induces the unfolded protein response in PSGs of Nd-s° strain, which will be a valuable tool for protein quality-control studies of silk grand.
机译：丝素是蚕丝纤维的主要成分，在家蚕中由轻（L）链、重链和fhx/P25组成。丝胶茧（Nd-s°）是一种表达突变丝素L链的家蚕品系，该突变丝素L链积聚在后丝腺（PSG）的内质网（ER）中。然而，对于Nd-s~D菌株中PSG的积累效应知之甚少。我们比较了Nd-s~D株和产生丝素的正常株5龄幼虫的PSG基因表达谱。cDNA代表性差异分析确定了候选基因，其在Nd-s°菌株中的表达水平高于正常菌株。我们主要研究热休克蛋白和组织蛋白酶B，一种主要的溶酶体蛋白酶。我们证实了BIP（GRP78）和Hsp20的上调。8和组织蛋白酶B在转录和/或翻译水平。这些结果表明，突变体L链的积累在Nd-s°菌株的PSG中诱导了未折叠的蛋白质反应，这将是一种有价值的丝绸品质控制研究工具。

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