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首页> 外国专利> METHOD AND USES FOR BOMBYX MORI SILK FIBROIN HEAVY CHAIN GENE MUTATION SEQUENCE AND MUTANT

METHOD AND USES FOR BOMBYX MORI SILK FIBROIN HEAVY CHAIN GENE MUTATION SEQUENCE AND MUTANT

机译：BOMBYX MORI丝蛋白重链基因突变序列和突变体的方法和用途

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摘要

Provided is a method for Bombyx mori silk fibroin heavy chain gene mutation, specifically comprising causing the mRNA of a coded zinc finger nuclease sequence to act upon a target point such as positions 1325-1362 of Bombyx mori silk fibroin heavy chain gene as expressed in SEQ ID NO:1, so as to obtain a series of Bombyx mori silk fibroin heavy chain mutant genes. Also provided is a mutation sequence prepared according to said method. Also provided are uses of said mutation sequence in the preparation of sericin and foreign proteins. Also provided is a Bombyx mori mutant comprising the described mutation sequence as a novel Bombyx mori silk gland bioreactor, the rear silk gland of said mutant being severely degraded and the cocoon layer whereof including only sericin synthesized and excreted by the middle silk gland, whereby the expression volume and purity of foreign transgenes in the rear silk gland of Bombyx mori can be greatly increased and large-scale production of sericin can be achieved.
机译：提供了一种家蚕丝纤蛋白重链基因突变的方法，具体包括使编码的锌指核酸酶序列的mRNA作用于目标点，例如SEQ ID NO：1所示的家蚕丝纤蛋白重链基因的位置1325-1362。编号：1，以获得一系列家蚕丝素蛋白重链突变基因。还提供了根据所述方法制备的突变序列。还提供了所述突变序列在丝胶蛋白和外源蛋白的制备中的用途。还提供了家蚕突变体，其包含作为新家蚕丝绸腺体生物反应器描述的突变序列，所述突变体的后部丝绸腺体被严重降解，并且其茧层仅包括由中间丝腺体合成和排泄的丝胶。可以大大提高家蚕后部丝腺中外源转基因的表达量和纯度，并可以大规模生产丝胶蛋白。

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