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首页> 外文期刊>Journal of insect biotechnology and sericology >The physiological accumulation of mutant fibroin light chains induces an unfolded protein response in the posterior silk gland of the Sericin cocoon Nd-sD strain of silkworm Bombyx mori
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The physiological accumulation of mutant fibroin light chains induces an unfolded protein response in the posterior silk gland of the Sericin cocoon Nd-sD strain of silkworm Bombyx mori

机译：突变的丝蛋白轻链的生理积累诱导家蚕家蚕Sricin茧Nd-sD株后丝腺中未折叠的蛋白质反应

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摘要

Fibroin, a major component of silk fiber, is composed of light (L) chains, heavy chains, and fhx/P25 in the silkworm Bombyx mori . Sericin cocoon ( Nd-sD ) is a silkworm strain expressing a mutant fibroin L chain that is accumulated in the endoplasmic reticulum (ER) of posterior silk glands (PSGs). However, little is known about the effects of accumulation in PSGs in Nd-sD strain. We compared the PSG gene expression profiles of 5th-instar larvae of Nd-sD strain and the fibroin-producing normal strain. cDNA representational difference analysis identified candidate genes whose expression levels were higher in Nd-sD strain than in normal strain. We focused on heat shock proteins and cathepsin B, a major lysosomal protease. We confirmed upregulation of BiP(GRP78), Hsp20.8, and cathepsin B at the transcriptional and/or translational levels. These results suggest that the accumulation of mutant L chain induces the unfolded protein response in PSGs of Nd-sD strain, which will be a valuable tool for protein quality-control studies of silk grand.
机译：丝素蛋白是蚕丝纤维的主要成分，由家蚕中的轻链（L），重链和fhx / P25组成。丝胶茧（Nd-s D ）是一种蚕菌株，表达突变型丝蛋白L链，该丝蛋白L链积聚在后丝腺（PSG）的内质网（ER）中。然而，对于Nd-s D 菌株中PSG中积累的影响知之甚少。我们比较了Nd-s D 菌株的五龄幼虫和产生丝蛋白的正常菌株的PSG基因表达谱。 cDNA代表性差异分析鉴定了Nd-s D 菌株中表达水平高于正常菌株的候选基因。我们专注于热休克蛋白和组织蛋白酶B，一种主要的溶酶体蛋白酶。我们证实BiP（GRP78），Hsp20.8和组织蛋白酶B在转录和/或翻译水平上调。这些结果表明，突变体L链的积累诱导了Nd-s D 菌株PSG中未折叠的蛋白质反应，这将成为丝绸大分子蛋白质质量控​​制研究的有价值的工具。

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