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首页> 外文期刊>Animal Feed Science and Technology >A novel method to determine simultaneously methane production during in vitro gas production using fully automated equipment.
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A novel method to determine simultaneously methane production during in vitro gas production using fully automated equipment.

机译：一种使用全自动设备确定体外气体生产过程中甲烷生产量的新方法。

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摘要

An adaptation of fully automated gas production equipment was tested for its ability to simultaneously measure methane and total gas. The simultaneous measurement of gas production and gas composition was not possible using fully automated equipment, as the bottles should be kept closed during the incubations. A separate small opening with a screw cap and septum was made in each bottle, making it possible to take very small aliquots (10 micro l) from the gas in the headspace with a syringe for immediate gas analysis. As the used automatic gas production equipment was a venting system, corrections had to be made for the vented total gas and methane, as well as for the dilution of the produced methane with the gas in the headspace. To test the suitability and accuracy of the system, known amounts of methane were injected in bottles in the venting system and methane concentrations in the headspace were determined. It proved that the methane concentration in the headspace, corrected for the vented gas, coincided with the injected amount of methane. To show the potency of the adapted equipment, experiments were conducted with different feedstuffs. Total gas production and methane production were recorded and their relationships were calculated. The ability of the system to test feed additives for methane reduction was demonstrated for maize and soybean hulls as substrate (0.5 g DM), supplemented with monensin (15 mg), sodium-2-bromoethanesulphonate (BES, 15 mg), cinnemaldehyde (150 mg) and tea tannins (150 mg), additives known to effect methane synthesis. The adapted gas production equipment showed to be a powerful tool to determine rate and extent of gas production as a measure of fermentation and to simultaneously determine methane production.
机译：测试了一套适应性强的全自动天然气生产设备的同时测量甲烷和总气体的能力。使用全自动设备无法同时测量产气量和产气量，因为在培养过程中应保持瓶盖密闭。在每个瓶中都制作了一个带有螺帽和隔垫的单独小开口，从而可以使用注射器从顶空中的气体中取出非常小的等分试样（10微升），以便立即进行气体分析。由于使用的自动气体生产设备是通风系统，因此必须对已排放的总气体和甲烷进行校正，并对顶空气体对产生的甲烷的稀释进行校正。为了测试系统的适用性和准确性，将已知量的甲烷注入通风系统的瓶中，并确定顶部空间的甲烷浓度。事实证明，校正后的顶部气体中的甲烷浓度已与排出的甲烷量相吻合。为了显示改造后的设备的效能，对不同的饲料进行了实验。记录天然气的总产量和甲烷的产量，并计算它们之间的关系。以玉米和大豆壳为底物（0.5 g DM），并补充莫能菌素（15 mg），2-溴乙烷磺酸钠（BES，15 mg）和肉桂醛（150），证明了该系统测试饲料添加剂降低甲烷的能力。毫克）和茶单宁（150毫克），已知会产生甲烷的添加剂。调整后的产气设备显示出是确定产气量和发酵程度并同时确定甲烷产量的有力工具。

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