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首页> 外文期刊>Journal of mass spectrometry: JMS >MALDI‐MS argininyl bufadienolide esters fingerprint from parotoid gland secretions of Rhinella arenarum Rhinella arenarumRhinella arenarum : Age, gender, and seasonal variation
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MALDI‐MS argininyl bufadienolide esters fingerprint from parotoid gland secretions of Rhinella arenarum Rhinella arenarumRhinella arenarum : Age, gender, and seasonal variation

机译：MALDI-MS Agininyl Bufdienolide酯来自拍摄腺体分泌物 rhinella arenarum 犀牛arenarum：年龄，性别和季节性变化

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Abstract > In many amphibians, the granular glands can be grouped in special regions forming macroglands. This is the case of toads, characterized by the presence of a pair of parotoid macroglands, strategically located to give protection by poison release in case of attacks. The product secreted consists of a wide variety of chemical compounds including proteins, peptides, biogenic amines, toxic steroidal bufadienolides, and various alkaloids, depending on the species. In this work, using <fc> <fi>Rhinella arenarum</fi> , </fc> we have performed, for the first time, the matrix assisted‐ultraviolet laser desorption/ionization mass spectrometry and tandem mass spectrometry characterization of the components of the secretion used as crude material, just suspended in MeOH (or MeCN). The crude sample as a whole (whole suspension) was spotted on the matrix assisted‐ultraviolet laser desorption plate for analysis. Electrospray ionization‐Orbitrap was used for cross‐checking experiments. The pattern of signals obtained at m/z ranges 600 to 800 and 1200 to 1600 could be assigned as the argininyl bufadienolide esters fingerprint characteristic of female and male. Variation patterns for gender (female, male), age (non‐reproductive, reproductive), and season (non‐reproductive, reproductive) are described. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><abstract xmlns=”http://www.wiley.com/namespaces/wiley“type=“main”xml:lang=“en”><title type=“main”“>摘要>在许多两栖动物中，颗粒腺体可分为特殊区域，形成大腺体。这是蟾蜍的情况，其特征是存在一对腮腺状大腺体，位于战略位置，以便在攻击时通过释放毒素提供保护。分泌的产物由多种化合物组成，包括蛋白质、peptIDE、生物胺、有毒甾体蟾蜍二烯内酯和各种生物碱，取决于物种。在这项工作中，我们首次利用莱茵菌进行基质辅助紫外激光解吸/电离质谱和串联质谱分析，对用作粗物质的分泌物成分进行了表征，这些成分仅悬浮在MeOH（或MeCN）中。将粗样品作为一个整体（整个悬浮液）放置在基质辅助紫外激光解吸板上进行分析。电喷雾电离Orbitrap用于交叉检查实验。在m/z范围600至800和1200至1600处获得的信号模式可被指定为女性和男性的精氨酰丁二烯内酯酯指纹特征。描述了性别（女性、男性）、年龄（非生殖性、生殖性）和季节（非生殖性、生殖性）的变化模式 </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-34906/'>《Journal of mass spectrometry: JMS》</a> <b style="margin: 0 2px;">|</b><span>2018年第6期</span><b style="margin: 0 2px;">|</b><span>共11页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Petroselli Gabriela&option=202" target="_blank" rel="nofollow">Petroselli Gabriela;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Raices Marilina&option=202" target="_blank" rel="nofollow">Raices Marilina;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Jungblut Lucas D.&option=202" target="_blank" rel="nofollow">Jungblut Lucas D.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Pozzi Andrea G.&option=202" target="_blank" rel="nofollow">Pozzi Andrea G.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Erra‐Balsells Rosa&option=202" target="_blank" rel="nofollow">Erra‐Balsells Rosa;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Departamento de Química OrgánicaUniversidad de Buenos Aires. Facultad de Ciencias Exactas y NaturalesPabellón II 3er P. Ciudad Universitaria 1428 Buenos Aires Argentina;</p> <p>Facultad de Ciencias Exactas y NaturalesCONICET Universidad de Buenos Aires. Instituto de Biodiversidad y Biología Experimental y Aplicada (IBBEA)Pabellón II 4to P. Ciudad Universitaria 1428 Buenos Aires Argentina;</p> <p>Facultad de Ciencias Exactas y NaturalesCONICET Universidad de Buenos Aires. Instituto de Biodiversidad y Biología Experimental y Aplicada (IBBEA)Pabellón II 4to P. Ciudad Universitaria 1428 Buenos Aires Argentina;</p> <p>Facultad de Ciencias Exactas y NaturalesCONICET Universidad de Buenos Aires. Instituto de Biodiversidad y Biología Experimental y Aplicada (IBBEA)Pabellón II 4to P. Ciudad Universitaria 1428 Buenos Aires Argentina;</p> <p>Departamento de Química OrgánicaUniversidad de Buenos Aires. Facultad de Ciencias Exactas y NaturalesPabellón II 3er P. Ciudad Universitaria 1428 Buenos Aires Argentina;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/1187.html" title="分析化学">分析化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=fingerprint&option=203" rel="nofollow">fingerprint;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=MALDI‐MS&option=203" rel="nofollow">MALDI‐MS;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=bufadienolides&option=203" rel="nofollow">bufadienolides;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=parotoid secretion&option=203" rel="nofollow">parotoid secretion;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Rhinella arenarum&option=203" rel="nofollow">Rhinella arenarum;</a> </p> <div class="translation"> 机译：None; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704025633326.html">MALDI‐MS argininyl bufadienolide esters fingerprint from parotoid gland secretions of <fc xmlns="http://www.wiley.com/namespaces/wiley"> <fi>Rhinella arenarum</fi> </fc> <fi xmlns="http://www.wiley.com/namespaces/wiley">Rhinella arenarum</fi>Rhinella arenarum : Age, gender, and seasonal variation</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Petroselli Gabriela&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Petroselli Gabriela,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Raices Marilina&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Raices Marilina,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Jungblut Lucas D.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Jungblut Lucas D.,</a> <a href="/journal-foreign-34906/" target="_blank" rel="nofollow" class="tuijian_authcolor">Journal of mass spectrometry: JMS .</a> <span>2018</span><span>,第6期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: 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