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首页> 外文期刊>Journal of Medical Entomology >Dietary Effects on the Development of Calliphora dubia and Chrysomya rufifacies (Diptera: Calliphoridae): Implications for Postmortem Interval
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Dietary Effects on the Development of Calliphora dubia and Chrysomya rufifacies (Diptera: Calliphoridae): Implications for Postmortem Interval

机译：关于Calliphora Dubia和Chrysomya Rufifacies的饮食影响（Diptera：Calliphoridae）：对淘汰后的间隔的影响

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摘要

Forensic entomology relies on insect development data generated within a laboratory setting in the estimation of minimum postmortem interval (mPMI). The methodologies used to produce these data vary considerably within the field and there is no accepted standard approach to laboratory rearing of forensically relevant species. A wide range of rearing media are used across published studies, including different species of animal and types of tissue (e.g., muscle and liver). Differing methodologies, particularly rearing diet, can introduce considerable variation into the baseline data upon which forensic estimates of the mPMI are calculated. Consequently, research establishing a widely available, standard and/or optimal, rearing medium for blow fly development for forensic application is desirable. This study examined dietary effects on the development of two forensically relevant blow fly species: Calliphora dubia Macquart, 1855, and Chrysomya rufifacies Macquart 1842 (Diptera: Calliphoridae). Larvae of both species were reared on pork liver, pork mince, pork loin, beef liver, beef mince, and guinea pig carcass under two constant temperature regimes (24 +/- 1 degrees C and 30 +/- 1 degrees C; 70 +/- 10% humidity; 12-h/12-h photoperiod) to assess the influence of temperature on developmental response to diet. Fundamental developmental data pertaining to both species are reported. Developmental response to diet was species-specific and influenced by temperature with indication that the optimal temperature for C. dubia development is below 30 degrees C. Pork mince was the most appropriate dietary standard of the rearing media investigated for the formulation of forensic development data for both species investigated.
机译：法医昆虫学依靠实验室环境中产生的昆虫发育数据来估计最小死后间隔（mPMI）。用于产生这些数据的方法在现场有很大差异，没有公认的实验室饲养法医相关物种的标准方法。在已发表的研究中使用了广泛的饲养媒介，包括不同种类的动物和不同类型的组织（如肌肉和肝脏）。不同的方法，尤其是饲养饮食，可能会给基线数据带来相当大的变化，据此计算mPMI的法医学估计。因此，研究建立一种广泛可用的、标准的和/或最佳的、用于法医应用的苍蝇培养基是可取的。这项研究考察了饮食对两种法医学上相关的蝇类物种发育的影响：1855年的丽蝇和1842年的金蝇（双翅目：丽蝇科）。在两种恒温条件下（24+/-1摄氏度和30+/-1摄氏度；70+/-10%湿度；12小时/12小时光周期），在猪肝、猪肉糜、猪里脊、牛肉肝、牛肉糜和豚鼠尸体上饲养这两个物种的幼虫，以评估温度对饮食发育反应的影响。报告了这两个物种的基本发育数据。对饮食的发育反应具有物种特异性，并受温度的影响，表明杜氏梭菌发育的最佳温度低于30摄氏度。猪肉糜是研究的饲养介质中最合适的饮食标准，用于制定两个研究物种的法医发育数据。

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