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首页> 外文期刊>Journal of Neuroscience Methods >Tracking the fate and migration of cells in live animals with cell-cycle indicators and photoconvertible proteins
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Tracking the fate and migration of cells in live animals with cell-cycle indicators and photoconvertible proteins

机译：用细胞周期指示剂和可光电蛋白质跟踪活动物中细胞的命运和迁移

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摘要

Cell migration and cell proliferation are the basic principles that make up a living organism, and both biologically and medically. In order to understand living organism and biological phenomena, it is essential to track the migration, proliferation, and fate of cells in living cells and animals and to clarify the properties and molecular expression of cells. Recent developments in novel fluorescent proteins have made it possible to observe cell migration and proliferation as the cell cycle at the single-cell level in living individuals and tissues. Here, we introduce cell cycle visualization of living cells and animals by Fucci (Fluorescent Ubiquitination-based Cell Cycle Indicator) system and in situ cell labeling of cells and tracking cell migration by photoactivatable and photoconvertible proteins. In addition, we will present our established methods as an example of combines above tools with single-cell molecular expression analysis to reveal the fate of migrating cells at single cell level.
机译：细胞迁移和细胞增殖是构成生物体的基本原理，在生物学和医学上都是如此。为了理解生物体和生物现象，有必要追踪细胞在活细胞和动物中的迁移、增殖和命运，并阐明细胞的性质和分子表达。新型荧光蛋白的最新进展使得在活体个体和组织的单细胞水平上观察细胞迁移和增殖作为细胞周期成为可能。在这里，我们介绍了通过Fucci（基于荧光泛素化的细胞周期指示剂）系统对活细胞和动物的细胞周期可视化，以及细胞的原位细胞标记，并通过光激活和光转换蛋白质跟踪细胞迁移。此外，我们将以上述工具与单细胞分子表达分析相结合的方法为例，在单细胞水平上揭示迁移细胞的命运。

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