...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Parasitology >AN IMPROVED PCR PROTOCOL FOR DETECTION OF BABESIA DUNCANI IN WILDLIFE AND VECTOR SAMPLES
【24h】

AN IMPROVED PCR PROTOCOL FOR DETECTION OF BABESIA DUNCANI IN WILDLIFE AND VECTOR SAMPLES

机译：一种改进的PCR方案，用于检测野生动物和载体样品中的Babesia Duncani

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Human babesiosis is a tick-borne protozoal disease of increasing clinical significance in North America. Most cases in the eastern and Midwestern regions of the United States are reportedly due to Babesia microti infections. By contrast, most human infections reported in California and Washington have been attributed to a new species that was first identified in 1991 and subsequently named Babesia duncani. Although the tick vector and mammalian reservoir hosts for B. microti are well characterized, the vector and reservoir hosts for B. duncani are unknown. As a result, specific risk factors for human infections cannot be characterized. Identification of potential hosts and vector species has been hampered by the lack of specific and sensitive molecular diagnostic tools to amplify parasite DNA. To address this need, a nested PCR assay targeting the beta-tubulin gene, a well-conserved locus in piroplasm parasites with a highly variable intron region among species, was developed. The assay was evaluated by spiking tick and mammalian DNA extracts with DNA from a B. duncani isolate derived from a human patient (WA-1) as well as related Babesia spp. from Californian wildlife. This assay was highly specific, with a sensitivity of approximately 1 copy of template DNA in a background of tick DNA. At this level of detection B. duncani was detectable in larval tick samples, and the target locus allowed for visual differentiation between species by gel electrophoresis. This assay offers researchers a new tool for elucidating the natural transmission cycle of B. duncani.
机译：人类巴贝斯虫病是一种蜱传播的原生动物疾病，在北美具有越来越重要的临床意义。据报道，美国东部和中西部地区的大多数病例是由微小巴贝斯虫感染引起的。相比之下，在加利福尼亚和华盛顿报告的大多数人类感染都归因于1991年首次发现的一个新物种，后来被命名为邓卡巴贝斯虫。尽管微小双歧杆菌的蜱虫载体和哺乳动物宿主的特征很好，但邓肯双歧杆菌的载体和宿主尚不清楚。因此，无法描述人类感染的特定风险因素。由于缺乏特异和敏感的分子诊断工具来扩增寄生虫DNA，潜在宿主和载体物种的鉴定一直受到阻碍。为了满足这一需求，开发了一种针对β-微管蛋白基因的巢式PCR检测方法。β-微管蛋白基因是梨形虫寄生虫中一个高度保守的基因座，在物种间具有高度可变的内含子区域。通过将蜱虫和哺乳动物DNA提取物与来自人类患者（WA-1）的邓卡氏双歧杆菌分离物的DNA以及来自加利福尼亚野生动物的相关巴贝斯虫属进行加标来评估该检测。该分析具有高度特异性，在蜱虫DNA背景下，其灵敏度约为1份模板DNA。在这种检测水平下，在幼虫蜱样本中可检测到邓肯氏双歧杆菌，目标位点允许通过凝胶电泳进行物种间的视觉区分。该分析为研究人员提供了一种新的工具，用于阐明邓肯氏双歧杆菌的自然传播周期。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号